Chemical Biology Graduate Program, Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-2216, USA.
ACS Chem Biol. 2010 Jan 15;5(1):47-62. doi: 10.1021/cb900258z.
Hydrogen peroxide (H(2)O(2)) acts as a second messenger that can mediate intracellular signal transduction via chemoselective oxidation of cysteine residues in signaling proteins. This Review presents current mechanistic insights into signal-mediated H(2)O(2) production and highlights recent advances in methods to detect reactive oxygen species (ROS) and cysteine oxidation both in vitro and in cells. Selected examples from the recent literature are used to illustrate the diverse mechanisms by which H(2)O(2) can regulate protein function. The continued development of methods to detect and quantify discrete cysteine oxoforms should further our mechanistic understanding of redox regulation of protein function and may lead to the development of new therapeutic strategies.
过氧化氢 (H₂O₂) 作为一种第二信使，能够通过信号蛋白中半胱氨酸残基的化学选择性氧化来介导细胞内信号转导。本文综述了目前关于信号介导的 H₂O₂ 产生的机制见解，并重点介绍了近年来在体外和细胞内检测活性氧 (ROS) 和半胱氨酸氧化的方法的最新进展。本文选用了来自近期文献的一些实例来说明 H₂O₂ 调节蛋白质功能的多种机制。不断开发用于检测和定量离散半胱氨酸氧化形式的方法，应该可以增进我们对蛋白质功能氧化还原调节的机制理解，并可能导致新的治疗策略的发展。
