Veterinary Epidemiology Unit, Department of Obstetrics, Reproduction, and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke 820, Belgium.
Microb Drug Resist. 2010 Mar;16(1):67-74. doi: 10.1089/mdr.2009.0062.
This study explored the prevalence and persistence of acquired antimicrobial resistance in Escherichia coli and Enterococcus faecium from healthy broilers. In 32 broiler farms, cloacal samples were taken during two production rounds, with one production round in between. For 10 of the sampled flocks, samples from the carcasses at the slaughterhouse were also collected. For E. coli, high levels of resistance were found for ampicillin, nalidixic acid, streptomycin, tetracycline, and the combination of trimethoprim and sulfonamide. Over 58% of all the isolates showed resistance to four or more antimicrobial agents. Only 4.8% were fully susceptible for all 14 drugs tested. A remarkably high resistance rate (up to 41%) to ceftiofur was found. The enterococci were frequently resistant to macrolides, tetracycline, and the combination quinopristin/dalfopristin. Over 80% displayed acquired resistance to four or more antimicrobial agents, and 3.9% were fully susceptible for the eight agents tested. Resistance was found to persist over consecutive production rounds. There was a good correlation between results obtained with cloacal samples of the live animals and caecal content samples collected in the slaughterhouse for both E. coli and E. faecium. For E. coli but not for E. faecium, the resistance profile of neck skin isolates was different from that of cloacal isolates.
本研究探讨了健康肉鸡中大肠杆菌和粪肠球菌获得性抗药性的流行和持续情况。在 32 个肉鸡养殖场中，在两个生产轮次期间采集了泄殖腔样本，中间有一个生产轮次。对于 10 个采样的鸡群，还从屠宰场的胴体中采集了样本。对于大肠杆菌，发现对氨苄西林、萘啶酸、链霉素、四环素和甲氧苄啶-磺胺甲恶唑的组合有很高的耐药水平。超过 58%的所有分离株对四种或更多种抗菌药物表现出耐药性。只有 4.8%的分离株对测试的 14 种药物完全敏感。头孢噻呋的耐药率高达 41%。肠球菌经常对大环内酯类、四环素和奎奴普丁/达福普汀的组合耐药。超过 80%的分离株对四种或更多种抗菌药物表现出获得性耐药，对测试的 8 种药物中有 3.9%的分离株完全敏感。耐药性在连续的生产轮次中持续存在。对于大肠杆菌和粪肠球菌，从活禽泄殖腔样本中获得的结果与在屠宰场收集的盲肠内容物样本之间存在良好的相关性。但对于粪肠球菌而言，并非对于大肠杆菌，颈部皮肤分离株的耐药谱与泄殖腔分离株不同。
