Special Bacteriology, Emerging Bacterial Pathogens Division, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg R3E 3R2, Canada.
Eur J Clin Microbiol Infect Dis. 2010 Feb;29(2):191-205. doi: 10.1007/s10096-009-0840-3. Epub 2009 Dec 4.
Legionella pneumophila (Lp) is a significant cause of nosocomial, community-acquired, and travel-associated pneumonia in industrialized regions. Legionellosis has been a nationally notifiable disease in Canada since 1986, with an average of 75 cases reported annually; however, only the most severe, and often fatal, cases are reported or investigated. Here, epidemiological relationships, types, and distribution of Lp referrals to the Canadian national reference center were studied. Lp strains from different years, sources, and geographic locations were subtyped using a sequence-based typing (SBT) scheme and by the 'Joly' and/or 'Dresden' monoclonal antibody panels. Included were 128 epidemiologically unrelated clinical and 86 unrelated environmental strains. Sixty-four (index of diversity [IOD] = 0.964) and 45 (IOD = 0.888) sequence types (STs) were observed among clinical and environmental sources, respectively. Serogroup (sg) 1 was represented by 60.2% (77/128) and 52.3% (45/86) of clinical and environmental strains, respectively, and 63.6% (49/77) and 15.6% (7/45) of those were mAb2-positive, respectively. Serogroup 1, ST1 accounted for 14.1% (18/128) and 30.2% (26/86) of unrelated clinical and environmental isolates, respectively. This database will serve as a basis for Canadian epidemiological surveillance efforts and is linked to global surveillance initiatives curated by the European Working Group for Legionella Infections (EWGLI) network.
嗜肺军团菌（Lp）是工业化地区医院获得性、社区获得性和旅行相关性肺炎的重要病因。自 1986 年以来，军团病在加拿大已被列为国家法定报告疾病，每年平均报告 75 例病例；然而，只有最严重且经常致命的病例才会被报告或调查。在此，研究了嗜肺军团菌向加拿大国家参考中心报告的流行病学关系、类型和分布。使用基于序列的分型（SBT）方案和“Joly”和/或“Dresden”单克隆抗体面板对来自不同年份、来源和地理位置的嗜肺军团菌菌株进行了亚型分型。包括 128 株流行病学无关的临床株和 86 株无关的环境株。在临床和环境来源中分别观察到 64（多样性指数[IOD] = 0.964）和 45（IOD = 0.888）种序列型（ST）。血清群（sg）1 分别代表 60.2%（77/128）和 52.3%（45/86）的临床和环境菌株，其中 63.6%（49/77）和 15.6%（7/45）为 mAb2 阳性。血清群 1、ST1 分别占无关临床和环境分离株的 14.1%（18/128）和 30.2%（26/86）。该数据库将作为加拿大流行病学监测工作的基础，并与欧洲军团菌感染工作组（EWGLI）网络管理的全球监测倡议相关联。
