Section of Periodontology, Department of Operative Dentistry, University Hospital Heidelberg, Heidelberg, Germany.
J Periodontol. 2009 Dec;80(12):2020-7. doi: 10.1902/jop.2009.090241.
The identification of Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans) serotypes may add some important information to the understanding of the pathogenetic background of severe periodontal infections. This study compared serotypes of A. actinomycetemcomitans in two groups of periodontal patients with different ethnic backgrounds.
A total of 194 patients (96 Germans and 98 Koreans) with aggressive or severe chronic periodontitis participated in the study. Microbiologic analysis of pooled samples from subgingival plaque was performed by using a real-time polymerase chain reaction (PCR) test for A. actinomycetemcomitans. In patients who tested positive for A. actinomycetemcomitans, serotypes (a through f) were determined by nucleic acid-based methods.
The prevalence of patients who tested positive for A. actinomycetemcomitans with the real-time PCR was comparable in both groups (Germans: 27.0%; Koreans: 22.2%). In German patients, the serotypes detected most frequently were b (33.3%), c (25.0%), and a (20.8%), whereas in Korean patients, the serotype distribution was different, with serotypes c (61.9%) and d (19.0%) accounting for >80% of the complete serotype spectrum.
Even if the percentage of patients who tested positive for A. actinomycetemcomitans was identical in patients with generalized aggressive and severe chronic periodontitis and different ethnic backgrounds, the distribution of A. actinomycetemcomitans serotypes may exhibit marked differences.
鉴定伴放线放线杆菌（以前称为动弯杆菌伴放线放线杆菌）血清型可能会为严重牙周感染的发病机制背景增加一些重要信息。本研究比较了具有不同种族背景的两组牙周病患者中伴放线放线杆菌的血清型。
共有 194 名患有侵袭性或严重慢性牙周炎的患者（96 名德国人和 98 名韩国人）参加了这项研究。使用实时聚合酶链反应（PCR）试验对龈下菌斑的混合样本进行微生物分析，以检测伴放线放线杆菌。在检测到伴放线放线杆菌阳性的患者中，通过核酸为基础的方法确定血清型（a 至 f）。
实时 PCR 检测到伴放线放线杆菌阳性的患者在两组中的患病率相当（德国人：27.0%；韩国人：22.2%）。在德国患者中，检测到的最常见血清型为 b（33.3%）、c（25.0%）和 a（20.8%），而在韩国患者中，血清型分布不同，血清型 c（61.9%）和 d（19.0%）占完整血清型谱的>80%。
即使具有广泛侵袭性和严重慢性牙周炎以及不同种族背景的患者中检测到伴放线放线杆菌阳性的患者百分比相同，伴放线放线杆菌血清型的分布也可能存在明显差异。
