Division of Periodontology, Diagnostic Sciences & Dental Hygiene, Herman Ostrow School of Dentistry, University of Southern California, Los Angeles, CA 90089, USA.
Mol Oral Microbiol. 2010 Jun;25(3):207-14. doi: 10.1111/j.2041-1014.2010.00567.x.
This study examined the distribution pattern of Aggregatibacter actinomycetemcomitans serotypes in the subgingival plaque of subjects residing in the United States. A. actinomycetemcomitans was identified in 256 subgingival plaque samples from 161 subjects. For 190 of the 256 samples, the total cultivable bacteria and selected periodontal pathogenic species were determined. A. actinomycetemcomitans isolates were confirmed by a16S rDNA-based PCR analysis, genotyped by arbitrarily-primed PCR, and serotyped by PCR analysis of serotype-specific gene clusters. A total of 82 distinct A. actinomycetemcomitans strains were identified. The serotype distribution pattern of the strains was 21 (25.6%) serotype a, 12 (14.6%) b, 41 (50%) c, 6 (7.3%) e, 1 (1.2%) f, and 1 (1.2%) non-typeable. For 14 subjects where multiple colonies of A. actinomycetemcomitans were identified, 11 subjects (78.6%) were each infected by a single serotype, while the remaining three subjects (21.3%) were each infected by two serotypes of A. actinomycetemcomitans. There was an inverse relationship between the level of cultivable A. actinomycetemcomitans and Porphyromonas gingivalis. Within subgingival plaque of study cohort A. actinomycetemcomitans serotype c was the dominant serotype and comprised 50% of all strains, followed by (in order of detection frequency) serotypes a and b. Serotypes d, e, and f strains were either not detected or less frequently found. Serotype distribution patterns of subgingival A. actinomycetemcomitans may vary among subjects of different race orethnicity.
本研究检测了居住在美国人群的龈下菌斑中伴放线放线杆菌（Aggregatibacter actinomycetemcomitans）血清型的分布模式。从 161 名受试者的 256 个龈下菌斑样本中鉴定出了伴放线放线杆菌。对于 256 个样本中的 190 个样本，确定了总可培养细菌和选定的牙周病原种。通过基于 16S rDNA 的 PCR 分析确认伴放线放线杆菌分离株，通过任意引物 PCR 进行基因分型，并通过针对血清型特异性基因簇的 PCR 分析进行血清型分型。共鉴定出 82 种不同的伴放线放线杆菌菌株。菌株的血清型分布模式为 21（25.6%）血清型 a、12（14.6%）b、41（50%）c、6（7.3%）e、1（1.2%）f 和 1（1.2%）非定型。对于 14 名受试者，其中鉴定出多个伴放线放线杆菌菌落，11 名受试者（78.6%）每人感染单一血清型，而其余 3 名受试者（21.3%）每人感染两种血清型的伴放线放线杆菌。可培养的伴放线放线杆菌水平与牙龈卟啉单胞菌呈负相关。在研究队列的龈下菌斑中，伴放线放线杆菌血清型 c 是主要血清型，占所有菌株的 50%，其次是（按检测频率顺序）血清型 a 和 b。血清型 d、e 和 f 菌株要么未检出，要么检出频率较低。龈下伴放线放线杆菌的血清型分布模式可能因不同种族或民族的受试者而异。
