Department of Orthopaedic Surgery, Hiroshima University, Hiroshima, Japan.
Arthroscopy. 2009 Dec;25(12):1435-41. doi: 10.1016/j.arthro.2009.06.009. Epub 2009 Nov 6.
The purpose of this study was to investigate whether it is possible to regenerate degenerated human cartilage in vitro by use of magnetically labeled mesenchymal stem cells (MSCs) and an external magnetic device.
MSCs from human bone marrow were cultured and magnetically labeled. Degenerated human cartilage was obtained during total knee arthroplasty. The osteochondral fragments were attached to the sidewall of tissue culture flasks, and magnetically labeled MSCs were injected into the flasks. By use of an external magnetic device, a magnetic force was applied for 6 hours to the direction of the cartilage, and then the degenerated cartilage was cultured in chondrogenic differentiation medium for 3 weeks. In the control group a magnetic force was not applied. The specimens were evaluated histologically.
A cell layer was formed on the degenerated cartilage as shown by H&E staining. The cell layer was also stained in toluidine blue and safranin O and with anti-collagen type II immunostaining, indicating that the cell layer contained an extracellular matrix. In the control group a cell layer was not observed on the cartilage.
We were able to show that our system could deliver MSCs onto degenerated human cartilage and then form an extracellular matrix on the degenerated cartilage in vitro.
Our novel cell delivery system using magnetic force may lead toward a new treatment option for osteoarthritis.
本研究旨在探讨利用磁标记间充质干细胞（MSCs）和外部磁装置是否可以在体外再生退变的人软骨。
培养并磁标记人骨髓来源的 MSCs。在全膝关节置换术中获得退变的人软骨。将骨软骨碎片附着在组织培养瓶的侧壁上，并将磁标记的 MSCs 注入瓶中。通过外部磁装置，将磁力沿软骨方向施加 6 小时，然后将退变的软骨在软骨分化培养基中培养 3 周。在对照组中，不施加磁力。对标本进行组织学评估。
H&E 染色显示退变软骨上形成了细胞层。甲苯胺蓝和番红 O 染色以及抗胶原 II 免疫染色也显示细胞层含有细胞外基质。在对照组中，软骨上未观察到细胞层。
我们能够表明，我们的系统可以将 MSCs 递送到退变的人软骨上，并在体外在退变的软骨上形成细胞外基质。
我们使用磁力的新型细胞输送系统可能为骨关节炎提供一种新的治疗选择。
