Swaim W R
Department of Laboratory Medicine, University of Minnesota School of Medicine, Minneapolis.
Am J Clin Pathol. 1991 Mar;95(3):381-8. doi: 10.1093/ajcp/95.3.381.
Eight hundred-cell manual white blood cell differential count evaluations of Coulter VCS and Technicon H-1 included estimates of accuracy, imprecision, random and systematic errors, clinical relevance, and detection of immature neutrophils. Accuracy was acceptable, except for H-1 testing of monocytes. Instrument imprecision was similar, except for tighter monocyte values as measured by the VCS. Deming regression determined random errors were greater for H-1 monocytes and proportional errors were similar. Constant errors for the VCS were less than the H-1 for neutrophils and monocytes and greater than the H-1 for eosinophils and lymphocytes. H-1 morphologic false-negative rates were twice those for VCS. True-positive and false-negative rates in cases with immature neutrophils were 70.6% and 20.3% for the VCS and 35% and 55.9% for the H-1. The reference false-positive rate was 4.4%. Clinically appropriate VCS flags were generated in distributionally abnormal cases. Predictive values were higher for the VCS.
对库尔特VCS和Technicon H-1进行的800个细胞的手工白细胞分类计数评估包括准确性、不精密度、随机误差和系统误差的估计、临床相关性以及未成熟中性粒细胞的检测。除了H-1对单核细胞的检测外，准确性是可以接受的。仪器不精密度相似，除了VCS测量的单核细胞值更紧密。德明回归确定H-1单核细胞的随机误差更大，比例误差相似。VCS的恒定误差对于中性粒细胞和单核细胞小于H-1，对于嗜酸性粒细胞和淋巴细胞大于H-1。H-1的形态学假阴性率是VCS的两倍。在存在未成熟中性粒细胞的病例中，VCS的真阳性率和假阴性率分别为70.6%和20.3%，H-1的分别为35%和55.9%。参考假阳性率为4.4%。在分布异常的病例中生成了临床上合适的VCS标记。VCS的预测值更高。
