Cembrowski G S, Wilson M K, Adelmeyer S G, Chang C K
Am J Clin Pathol. 1983 Sep;80(3):333-41. doi: 10.1093/ajcp/80.3.333.
The traditional approach to the evaluation of accuracy of discrete differential counters uses a graphic comparison of automated and manual counts. This type of comparison introduces so much sampling variation that significant differences often are obscured. To exclude this sampling variability, one of three technologists, a reference technologist and the Coulter diff3/50, each performed a differential count on exactly the same 100 cells of 47 normal and 56 abnormal blood smears. Linear regression analysis demonstrated excellent agreement between the technologists for all cell types with the exception of band neutrophils. Comparison of the instrument results with the technologists' results showed very good agreement for segmented neutrophils, lymphocytes, monocytes, and eosinophils. The identification of band neutrophils, basophils, immature granulocytes and atypical lymphocytes requires improvement. The overall performance is acceptable, with only 11 leukocytes, on the average normal smear, misclassified out of 100 normal leukocytes (four bands called segs, four segs called bands, and three non-neutrophils incorrectly classified). We recommend similar evaluations for all discrete differential counters.
评估离散型血细胞分类计数器准确性的传统方法是对自动计数和手工计数进行图形比较。这种比较引入了太多的抽样变异，以至于显著差异常常被掩盖。为排除这种抽样变异性，三名技术人员中的一名、一名参考技术人员以及库尔特diff3/50血细胞分析仪，分别对47份正常血涂片和56份异常血涂片上完全相同的100个细胞进行了分类计数。线性回归分析表明，除杆状核中性粒细胞外，所有细胞类型在技术人员之间的一致性都非常好。仪器结果与技术人员结果的比较显示，分叶核中性粒细胞、淋巴细胞、单核细胞和嗜酸性粒细胞的一致性非常好。杆状核中性粒细胞、嗜碱性粒细胞、未成熟粒细胞和非典型淋巴细胞的识别需要改进。总体性能是可以接受的，在平均正常涂片上，每100个正常白细胞中平均只有11个白细胞被错误分类（4个杆状核被误称为分叶核，4个分叶核被误称为杆状核，3个非中性粒细胞被错误分类）。我们建议对所有离散型血细胞分类计数器进行类似评估。
