Collins W E, Anders R F, Ruebush T K, Kemp D J, Woodrow G C, Campbell G H, Brown G V, Irving D O, Goss N, Filipski V K
Center for Infectious Diseases, Atlanta, Georgia.
Am J Trop Med Hyg. 1991 Jan;44(1):34-41. doi: 10.4269/ajtmh.1991.44.34.
Aotus nancymai were immunized with the 4-mer, 8-mer, and 11-mer repeat peptides of the ring-infected erythrocyte surface antigen molecule of Plasmodium falciparum conjugated to diphtheria toxoid with muramyl dipeptide (MDP) as adjuvant. Immunization failed to induce protective immunity against the Uganda Palo Alto strain of P. falciparum as judged by maximum levels of parasitemia of immunized monkeys relative to those of controls. The fused polypeptide FPAg632, when combined with MDP, also failed to induce protective immunity. However, the maximum level of parasitemia and serologic response to the 11-mer peptide were inversely correlated. The safety of the use of MDP was evident.
以南美夜猴（Aotus nancymai）为实验对象，用恶性疟原虫环感染红细胞表面抗原分子的4肽、8肽和11肽重复序列肽与白喉类毒素偶联，并以胞壁酰二肽（MDP）作为佐剂进行免疫。根据免疫猴子的最高血寄生虫水平与对照组相比来判断，免疫未能诱导出针对恶性疟原虫乌干达帕洛阿尔托株的保护性免疫。融合多肽FPAg632与MDP联合使用时，也未能诱导出保护性免疫。然而，11肽的最高血寄生虫水平与血清学反应呈负相关。使用MDP的安全性是显而易见的。
