Department of Chemical Engineering, National Taipei University of Technology, Taipei 106, Taiwan.
J Biomed Mater Res A. 2010 Jun 15;93(4):1463-9. doi: 10.1002/jbm.a.32643.
Fibroblasts alter their mode of attachment and focal contact when placed on square arrays of silicon pillars. The pillars had 1-microm diameters with identical surface chemistry. Distance between pillars is 9 microm and height of pillars is 1, 5, or 10 microm on substrates. We found that these micropillars, rather than specific interactions, provided more opportunities for mechanical interlocking of the fibroblasts and acted as physical barriers that restrained cell migration. The cellular morphology and behavior is guidable by the height of pillars. In some cases, the fibroblasts filled in the intervals among several pillars; in others, a pillar protruded visibly through the cell body but did not pierce it, the cells were survived. Therefore, fibroblasts were immobilized upon in situ and the cytoplasma migrated outward to the bottom of the substrate subsequently. Laminin plays a critical role in cell attachment to the basement membrane. The results of laminin expression in fibroblasts suggest that pillar pattern appears to change cellular behavior and affect laminin expression significantly.
成纤维细胞在置于具有相同表面化学性质的硅质方柱阵列上时，会改变其附着方式和焦点接触方式。这些柱子的直径为 1 微米，柱子之间的距离为 9 微米，柱子的高度为 1、5 或 10 微米。我们发现这些微柱，而不是特定的相互作用，为成纤维细胞的机械联锁提供了更多的机会，并起到了物理屏障的作用，限制了细胞的迁移。细胞的形态和行为可以通过柱子的高度来引导。在某些情况下，成纤维细胞填充在几个柱子之间的间隔中；在其他情况下，柱子明显突出穿过细胞体，但没有刺穿它，细胞存活下来。因此，成纤维细胞被固定在原位，细胞质随后向外迁移到底物的底部。层粘连蛋白在细胞附着到基底膜上起着至关重要的作用。成纤维细胞中层粘连蛋白表达的结果表明，柱子模式似乎改变了细胞行为，并显著影响层粘连蛋白的表达。
