Meyle J, Gültig K, Wolburg H, von Recum A F
Sonderforschungsbereich Implantology, Poliklinik für Zahnärztliche Chirurgie und Parodontologie, Tübingen, Germany.
J Biomed Mater Res. 1993 Dec;27(12):1553-7. doi: 10.1002/jbm.820271212.
The contact between tissue and the implanted biomaterial is influenced by the micromorphology of the implant surface as well as biomechanical reactions. This effect is mediated by subcellular morphological structures and can affect the anchorage of the material inside the body of the host. The aim of the present study was to ascertain by transmission electron microscopy how human gingival fibroblasts interact with surface events. A special replica technique was used to produce a line pattern of 1 micron pitch with a depth of 1 micron. It was demonstrated, by transmission electron microscopy, that cells seeded on this surface extended cellular processes into the grooves, leading to an intensive contact and probably to mechanical interlocking. The typical morphological structures at several points indicated the presence of focal adhesion sites.
组织与植入生物材料之间的接触受到植入物表面微观形态以及生物力学反应的影响。这种效应由亚细胞形态结构介导，并可影响材料在宿主体内的锚固。本研究的目的是通过透射电子显微镜确定人牙龈成纤维细胞如何与表面事件相互作用。采用一种特殊的复制技术制作了间距为1微米、深度为1微米的线纹图案。通过透射电子显微镜证明，接种在该表面的细胞将细胞突起延伸到凹槽中，导致紧密接触并可能形成机械互锁。几个点处的典型形态结构表明存在粘着斑位点。
