Department of Anatomy, Dankook University College of Medicine, Cheonan-si, San 29, Anseo-Dong, Cheonan, Chungnam, South Korea.
Brain Res. 2010 Feb 8;1313:232-41. doi: 10.1016/j.brainres.2009.11.079. Epub 2009 Dec 5.
Worldwide expansion of mobile phones and electromagnetic field (EMF) exposure has raised question of their possible biological effects on the brain and nervous system. Radiofrequency (RF) radiation might alter intracellular signaling pathways through changes in calcium (Ca(2+)) permeability across cell membranes. Changes in the expression of calcium binding proteins (CaBP) like calbindin D28-k (CB) and calretinin (CR) could indicate impaired Ca(2+)homeostasis due to EMF exposure. CB and CR expression were measured with immunohistochemistry in the hippocampus of mice after EMF exposure at 835 MHz for different exposure times and absorption rates, 1 h/day for 5 days at a specific absorption rate (SAR)=1.6 W/kg, 1 h/day for 5 days at SAR=4.0 W/kg, 5 h/day for 1 day at SAR=1.6 W/kg, 5 h/day for 1 day at SAR=4.0 W/kg, daily exposure for 1 month at SAR=1.6 W/kg. Body weights did not change significantly. CB immunoreactivity (IR) displayed moderate staining of cells in the cornu ammonis (CA) areas and prominently stained granule cells. CR IR revealed prominently stained pyramidal cells with dendrites running perpendicularly in the CA area. Exposure for 1 month produced almost complete loss of pyramidal cells in the CA1 area. CaBP differences could cause changes in cellular Ca(2+)levels, which could have deleterious effect on normal hippocampal functions concerned with neuronal connectivity and integration.
全球范围内手机的普及和电磁场（EMF）暴露引发了人们对于手机辐射可能对大脑和神经系统产生生物学效应的担忧。射频（RF）辐射可能通过改变细胞膜的钙离子（Ca(2+)）通透性来改变细胞内信号通路。钙结合蛋白（CaBP）如钙结合蛋白 D28k（CB）和钙视网膜蛋白（CR）的表达变化可能表明由于 EMF 暴露导致 Ca(2+)稳态受损。在 EMF 暴露于 835 MHz 不同暴露时间和吸收率后，通过免疫组织化学测量了小鼠海马中的 CB 和 CR 表达，吸收率为 1.6 W/kg 时每天暴露 1 小时，连续 5 天，吸收率为 4.0 W/kg 时每天暴露 1 小时，连续 5 天，吸收率为 1.6 W/kg 时每天暴露 5 小时，连续 1 天，吸收率为 4.0 W/kg 时每天暴露 5 小时，吸收率为 1.6 W/kg 时每月每天暴露。体重没有明显变化。CB 免疫反应性（IR）显示 CA 区细胞中度染色，颗粒细胞明显染色。CR IR 显示 CA 区垂直排列的树突的锥体细胞明显染色。暴露 1 个月后，CA1 区的锥体细胞几乎完全丢失。CaBP 差异可能导致细胞内 Ca(2+)水平的变化，这可能对与神经元连接和整合有关的正常海马功能产生有害影响。
