Bouillaguet Serge, Wataha John C, Zapata Oscar, Campo Marino, Lange Norbert, Schrenzel Jacques
Endodontics Unit, Section of Dental Medicine, University of Geneva, Geneva, Switzerland.
Photomed Laser Surg. 2010 Aug;28(4):519-25. doi: 10.1089/pho.2009.2505.
The aim of this study was to assess the ability of commonly available red- or blue-light dental sources to generate reactive oxygen species (ROS) from photosensitive chemicals that might be useful for photodynamic antimicrobial chemotherapy (PACT).
Although the use of red diode lasers is well documented, there is limited information on how useful blue-light sources might be for PACT in dental contexts.
A diode laser (Periowave; see Table 1 for material and equipment sources) emitting red light (660-675 nm) was used to activate toluidine blue; riboflavin and pheophorbide-a polylysine (pheophorbide-a-PLL) were photoactivated using an Optilux 501 curing unit emitting blue light (380-500 nm). Ozone gas (generated by OzoTop, Tip Top Tips, Rolle, Switzerland), sodium hypochlorite, and hydrogen peroxide were used for comparison. ROS production was estimated using an iodine-triiodide colorimetric assay, and ROS levels were plotted versus concentration of chemicals to determine each chemical's efficiency in ROS production. One-way ANOVA with Tukey post hoc analysis (alpha = 0.05) was used to compare the efficiencies of ROS production for the various chemicals.
Sodium hypochlorite, hydrogen peroxide, and ozone gas produced ROS spontaneously, whereas pheophorbide-a-PLL, riboflavin, and toluidine blue required light exposure. The efficiency of ROS production was higher for pheophorbide-a-PLL and toluidine blue than for ozone gas or riboflavin (p < 0.05). Hydrogen peroxide was the least efficient ROS producer.
The results of the current study support the use of blue- or red-light-absorbing photosensitizers as candidates to produce ROS for clinical applications. Blue-light photosensitizers were as efficient as red-light photosensitizers in producing ROS and more efficient than the oxidant chemicals currently used for dental disinfection.
本研究旨在评估常见的红光或蓝光牙科光源从光敏化学物质产生活性氧（ROS）的能力，这些光敏化学物质可能对光动力抗菌化学疗法（PACT）有用。
虽然红色二极管激光的使用已有充分记录，但关于蓝光光源在牙科环境中对PACT的有用程度的信息有限。
使用发射红光（660 - 675nm）的二极管激光（Periowave；材料和设备来源见表1）激活甲苯胺蓝；使用发射蓝光（380 - 500nm）的Optilux 501固化单元对核黄素和脱镁叶绿酸-a聚赖氨酸（脱镁叶绿酸-a - PLL）进行光激活。使用臭氧气体（由瑞士罗勒的OzoTop，Tip Top Tips公司产生）、次氯酸钠和过氧化氢作为对照。使用碘 - 三碘化物比色法估计ROS的产生，并绘制ROS水平与化学物质浓度的关系图，以确定每种化学物质产生ROS的效率。使用带有Tukey事后分析的单因素方差分析（α = 0.05）比较各种化学物质产生ROS的效率。
次氯酸钠、过氧化氢和臭氧气体自发产生ROS，而脱镁叶绿酸-a - PLL、核黄素和甲苯胺蓝需要光照。脱镁叶绿酸-a - PLL和甲苯胺蓝产生ROS的效率高于臭氧气体或核黄素（p < 0.05）。过氧化氢是产生ROS效率最低的物质。
本研究结果支持使用吸收蓝光或红光的光敏剂作为临床应用中产生ROS的候选物质。蓝光光敏剂在产生ROS方面与红光光敏剂一样有效，并且比目前用于牙科消毒的氧化化学物质更有效。
