Zhdanov V M, Bykovskiĭ A F, Emel'ianov B A, Shumkina O B, Zairov G K
Vopr Virusol. 1977 Jul-Aug(4):474-7.
Attempts were made to isolate and identify hepatitis A virus by the method of immune electron microscopy using also biophysical methods and the transfection procedure of cell cultures by nucleic acid preparations. In fecal specimens from patients with infectious hepatitis A, virus-like particles 25-30 nm in diameter were found which reacted with convalescent antisera and could be detected by the immune electron microscopy method. By the same method virus-like particles 22-25 nm in diameter were detected in the blood serum of the patients collected early in the disease. A method for their purification and concentration in cesium chloride density gradients has been developed. Attempts at transfection of the cell cultures with preparations of nucleic acids failed. In the course of the study, however, data were obtained which would subsequently facilitate isolation of the causative agent of hepatitis A.
人们尝试通过免疫电子显微镜法，并结合生物物理方法以及用核酸制剂转染细胞培养物的程序来分离和鉴定甲型肝炎病毒。在甲型传染性肝炎患者的粪便标本中，发现了直径为25 - 30纳米的病毒样颗粒，这些颗粒能与恢复期抗血清发生反应，并且可用免疫电子显微镜法检测到。用同样的方法，在疾病早期采集的患者血清中检测到了直径为22 - 25纳米的病毒样颗粒。已经开发出一种在氯化铯密度梯度中对其进行纯化和浓缩的方法。用核酸制剂转染细胞培养物的尝试失败了。然而，在研究过程中获得的数据随后将有助于甲型肝炎病原体的分离。
