Locarnini S A, Coulepis A G, Ferris A A, Lehmann N I, Gust I D
Intervirology. 1978;10(5):300-8. doi: 10.1159/000148992.
Hepatitis A virus was purified fecal specimens obtained from 2 patients with naturally acquired hepatitis A. The purification procedure involved differential centrifugation, organic solvent extraction, agarose gel filtration, ion-exchange chromatography, and isopycnic ultracentrifugation in cesium chloride. Using immune electron microscopy and discontinuous SDS-PAGE, this procedure was found to be effective in removing extraneous material from hepatitis A virus. There was significant recovery of virus as judged by immune electron microscopy and solid-phase radioimmunoassay. Using this protocol, it has been possible to obtain virus preparations of sufficient purity and high enough titer to enable biochemical studies to proceed.
从两名自然感染甲型肝炎的患者粪便标本中纯化甲型肝炎病毒。纯化过程包括差速离心、有机溶剂萃取、琼脂糖凝胶过滤、离子交换色谱以及在氯化铯中的等密度超速离心。通过免疫电子显微镜和不连续十二烷基硫酸钠-聚丙烯酰胺凝胶电泳发现，该方法可有效去除甲型肝炎病毒中的杂质。通过免疫电子显微镜和固相放射免疫测定法判断，病毒有显著回收。使用该方案，已能够获得纯度足够高且滴度足够高的病毒制剂，以使生化研究得以进行。
