Mok-Lin E, Ehrlich S, Williams P L, Petrozza J, Wright D L, Calafat A M, Ye X, Hauser R
The Fertility Center, Vincent Memorial Obstetrics and Gynecology, Massachusetts General Hospital, Boston, MA, USA.
Int J Androl. 2010 Apr;33(2):385-93. doi: 10.1111/j.1365-2605.2009.01014.x. Epub 2009 Nov 30.
Bisphenol A (BPA) is a synthetic chemical used in the manufacture of materials present in many common consumer products. In experimental animals, BPA caused oocyte aneuploidy and reduced production of oestradiol. In a prospective cohort study, we investigated the association between urinary BPA concentrations and ovarian response among women undergoing in vitro fertilization (IVF) at the Massachusetts General Hospital (MGH) Fertility Center. The geometric mean of two specific-gravity (SG) adjusted urinary BPA concentrations collected during each IVF cycle was used as the cycle-specific BPA exposure level. BPA concentrations were measured using online solid phase extraction coupled to isotope dilution-high-performance liquid chromatography-tandem mass spectrometry. Peak serum oestradiol was measured using the Elecsys Estradiol II immunoassay kit. Multivariable mixed effect models and Poisson regression models adjusting for correlation between multiple IVF cycles in the same woman were used to evaluate the association between urinary BPA concentrations and ovarian response, adjusting for age, BMI and day 3 follicle stimulating hormone (FSH) levels, a clinical measure of ovarian reserve. Urinary BPA concentrations were measured in 84 women (mean age 35.6 years) undergoing 112 IVF cycles; 23 women (27%) contributed more than one IVF cycle. BPA concentrations ranged from <0.4 to 25.5 microg/L (geometric mean 2.52 +/- SD 3.2); 15% of urine samples had concentrations <0.4 microg/L. Peak serum oestradiol levels correlated with the total number of oocytes retrieved per cycle (r = 0.65, p < 0.001). For each log unit increase in SG-BPA, there was an average decrease of 12% (95% CI: 4, 23%; p = 0.007) in the number of oocytes retrieved and an average decrease of 213 pg/ml (95% CI: -407, -20; p = 0.03) in peak oestradiol. BPA was detected in the urine of the majority of women undergoing IVF, and was inversely associated with number of oocytes retrieved and peak oestradiol levels.
双酚A（BPA）是一种合成化学物质，用于制造许多常见消费品中的材料。在实验动物中，双酚A导致卵母细胞非整倍体并降低雌二醇的产生。在一项前瞻性队列研究中，我们调查了马萨诸塞州总医院（MGH）生育中心接受体外受精（IVF）的女性尿液中双酚A浓度与卵巢反应之间的关联。每个IVF周期收集的两种比重（SG）调整后的尿液双酚A浓度的几何平均值用作特定周期的双酚A暴露水平。使用在线固相萃取结合同位素稀释-高效液相色谱-串联质谱法测量双酚A浓度。使用Elecsys Estradiol II免疫分析试剂盒测量血清雌二醇峰值。使用多变量混合效应模型和泊松回归模型，对同一女性多个IVF周期之间的相关性进行调整，以评估尿液双酚A浓度与卵巢反应之间的关联，并对年龄、体重指数和第3天卵泡刺激素（FSH）水平进行调整，这是卵巢储备的一项临床指标。对84名接受112个IVF周期的女性（平均年龄35.6岁）的尿液双酚A浓度进行了测量；23名女性（27%）参与了不止一个IVF周期。双酚A浓度范围为<0.4至25.5微克/升（几何平均值2.52±标准差3.2）；15%的尿液样本浓度<0.4微克/升。血清雌二醇峰值水平与每个周期回收的卵母细胞总数相关（r = 0.65，p < 0.001）。对于SG-BPA每增加一个对数单位，回收的卵母细胞数量平均减少12%（95%置信区间：4，23%；p = 0.007），雌二醇峰值平均减少213皮克/毫升（95%置信区间：-407，-20；p = 0.03）。在大多数接受IVF的女性尿液中检测到双酚A，并且它与回收的卵母细胞数量和雌二醇峰值水平呈负相关。
