College of Pharmacy, Core Research Institute, Chungbuk National University, 12, Gaesin-dong, Heungduk-gu, Cheongju, Chungbuk 361-763, Republic of Korea.
Pharmacol Biochem Behav. 2010 Mar;95(1):31-40. doi: 10.1016/j.pbb.2009.12.003. Epub 2009 Dec 11.
The components of the herb Magnolia officinalis have exhibited antioxidant and neuroprotective activities. In this study, we investigated effects of ethanol extract of M.officinalis and its major component 4-O-methylhonokiol on memory dysfunction and neuronal cell damages caused by A beta. Oral pretreatment of ethanol extract of M. officinalis (2.5, 5 and 10mg/kg) and 4-O-methylhonokiol (1mg/kg) into drinking water for 5 weeks suppressed the intraventricular treatment of A beta(1-42) (0.5 microg/mouse, i.c.v.)-induced memory impairments. In addition, 4-O-methylhonokiol prevented the A beta(1-42)-induced apoptotic cell death as well as beta-secretase expression. 4-O-methylhonokiol also inhibited H(2)O(2) and A beta(1-42)-induced neurotoxicity in cultured neurons as well as PC12 cells by prevention of the reactive oxygen species generation. 4-O-methylhonokiol also directly inhibited beta-secretase activity and A beta fibrilization in vitro. Thus, ethanol extract of M. officinalis may be useful for prevention of the development or progression of AD, and 4-O-methylhonokiol may be a major active component.
厚朴的成分表现出抗氧化和神经保护活性。在这项研究中，我们研究了厚朴乙醇提取物及其主要成分 4-O-甲基厚朴酚对 Aβ引起的记忆功能障碍和神经元细胞损伤的作用。厚朴乙醇提取物（2.5、5 和 10mg/kg）和 4-O-甲基厚朴酚（1mg/kg）通过口服预处理进入饮用水中 5 周，可抑制脑室注射 Aβ（1-42）（0.5μg/只，icv）引起的记忆损伤。此外，4-O-甲基厚朴酚可预防 Aβ（1-42）诱导的细胞凋亡以及β-分泌酶的表达。4-O-甲基厚朴酚还通过抑制活性氧的产生，抑制培养神经元和 PC12 细胞中 H2O2 和 Aβ（1-42）诱导的神经毒性。4-O-甲基厚朴酚还可以直接抑制β-分泌酶的活性和 Aβ的纤维化。因此，厚朴乙醇提取物可能对 AD 的发展或进展有用，4-O-甲基厚朴酚可能是主要的活性成分。
