Thayer School of Engineering, Dartmouth College, Hanover, NH, USA.
Int J Radiat Oncol Biol Phys. 2010 Jan 1;76(1):251-9. doi: 10.1016/j.ijrobp.2009.08.041.
A treatment monitoring study investigated the differential effects of orthotopic pancreatic cancer models in response to interstitial photodynamic therapy (PDT), and the validity of using magnetic resonance imaging as a surrogate measure of response was assessed.
Different orthotopic pancreatic cancer xenograft models (AsPC-1 and Panc-1) were used to represent the range of pathophysiology observed in human beings. Identical dose escalation studies (10, 20, and 40J/cm) using interstitial verteporfin PDT were performed, and magnetic resonance imaging with T2-weighted and T1-weighted contrast were used to monitor the total tumor volume and the vascular perfusion volume, respectively.
There was a significant amount of necrosis in the slower-growing Panc-1 tumor using high light dose, although complete necrosis was not observed. Lower doses were required for the same level of tumor kill in the faster-growing AsPC-1 cell line.
The tumor growth rate and vascular pattern of the tumor affect the optimal PDT treatment regimen, with faster-growing tumors being relatively easier to treat. This highlights the fact that therapy in human beings shows a heterogeneous range of outcomes, and suggests a need for careful individualized treatment outcomes assessment in clinical work.
一项治疗监测研究调查了原位胰腺癌模型对间质光动力疗法（PDT）的反应的差异影响，并评估了使用磁共振成像作为反应替代测量的有效性。
使用不同的原位胰腺癌异种移植模型（AsPC-1 和 Panc-1）来代表人类中观察到的病理生理学范围。使用间质维替泊芬 PDT 进行了相同的剂量递增研究（10、20 和 40J/cm），并使用 T2 加权和 T1 加权对比磁共振成像分别监测总肿瘤体积和血管灌注体积。
在生长较慢的 Panc-1 肿瘤中使用高光剂量会导致大量坏死，尽管未观察到完全坏死。在生长较快的 AsPC-1 细胞系中，需要较低的剂量才能达到相同水平的肿瘤杀伤。
肿瘤的生长速度和血管模式会影响最佳 PDT 治疗方案，生长较快的肿瘤相对更容易治疗。这突出表明，人类的治疗会产生不同的结果，这表明在临床工作中需要仔细评估个体化的治疗效果。
