Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Cantoblanco, 28049 Madrid, Spain.
Antiviral Res. 2010 Mar;85(3):556-8. doi: 10.1016/j.antiviral.2009.12.005. Epub 2009 Dec 18.
In previous work we have reported the immunization of swine using in vitro-transcribed foot-and-mouth disease virus (FMDV) RNA. With the aim of testing whether RNA-induced immunization can mediate protection against viral infection, a group of Swiss adult mice was inoculated with FMDV infectious transcripts. In most inoculated animals viral RNA was detected in serum at 48-72h postinoculation. A group of the RNA-inoculated mice (11 out of 19) developed significant titers of neutralizing antibodies against FMDV. Among those animals that were successfully challenged with infectious virus (15 out of 19), three out of the eight animals immunized upon RNA inoculation were protected, as infectious virus could not be isolated from sera but specific anti-FMDV antibodies could be readily detected. These results suggest the potential of the inoculation of genetically engineered FMDV RNA for virulence and protection assays in the murine model and allow to explore the suitability of RNA-based FMDV vaccination in natural host animals.
在之前的工作中，我们已经报告了使用体外转录的口蹄疫病毒（FMDV）RNA 对猪进行免疫接种。为了测试 RNA 诱导的免疫接种是否能介导对病毒感染的保护，一组瑞士成年小鼠用 FMDV 传染性转录本进行了接种。在大多数接种动物中，病毒 RNA 在接种后 48-72 小时在血清中被检测到。一组 RNA 接种的小鼠（19 只中的 11 只）产生了针对 FMDV 的中和抗体的显著滴度。在那些用传染性病毒成功挑战的动物中（19 只中的 15 只），在 RNA 接种时免疫的 8 只动物中有 3 只得到了保护，因为无法从血清中分离出传染性病毒，但可以很容易地检测到针对 FMDV 的特异性抗体。这些结果表明，在小鼠模型中接种遗传工程 FMDV RNA 进行毒力和保护试验具有潜力，并允许探索 RNA 为基础的 FMDV 疫苗接种在天然宿主动物中的适用性。
