State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, 6 Rong Jing Dong Jie, Beijing 100176, China.
Virology. 2010 Mar 1;398(1):57-67. doi: 10.1016/j.virol.2009.11.036. Epub 2009 Dec 14.
In order to investigate the global and dynamic host microRNAs (miRNAs)/messenger RNAs (mRNAs) expression alteration during in vitro acute HCV infection, a comprehensive microarray analysis was performed using human hepatoma cells. Totally, 108 human miRNAs and 1247 mRNAs were identified whose expression levels changed for more than 2.0-fold in response to HCV infection. Upon HCV infection, signature from the unique miRNA expression pattern reflected the involvement of miRNA-regulated host cellular physiology and antiviral mechanism, whereas a preponderance of differentially regulated genes associated with metabolism, cell growth, apoptosis and cytokine/chemokine pathways. Furthermore, a reverse regulatory association of differentially expressed miRNAs and their predicted targets was constructed. Finally, the differentially expressed miRNAs such as miR-24, miR-149, miR-638 and miR-1181 were identified to be involved in HCV entry, replication and propagation. These results suggest that combined miRNA and mRNA profiling may have superior potential as a diagnostic and mechanistic feature in HCV infection.
为了研究体外丙型肝炎病毒（HCV）感染过程中宿主微小 RNA（miRNA）/信使 RNA（mRNA）的整体和动态表达变化，我们使用人肝癌细胞进行了全面的微阵列分析。总共鉴定出 108 个人类 miRNA 和 1247 个 mRNA，它们的表达水平在 HCV 感染后发生了超过 2.0 倍的变化。在 HCV 感染后，独特的 miRNA 表达模式反映了 miRNA 调控宿主细胞生理和抗病毒机制的参与，而大量差异调节的基因与代谢、细胞生长、凋亡和细胞因子/趋化因子途径相关。此外，构建了差异表达 miRNA 与其预测靶基因的反向调控关联。最后，鉴定出差异表达的 miRNA 如 miR-24、miR-149、miR-638 和 miR-1181 参与 HCV 的进入、复制和传播。这些结果表明，miRNA 和 mRNA 联合分析可能具有作为 HCV 感染的诊断和机制特征的更大潜力。
