Department of Civil and Environmental Engineering, Michigan State University, East Lansing, Michigan 48824, USA.
Appl Environ Microbiol. 2010 Feb;76(3):956-9. doi: 10.1128/AEM.01364-09. Epub 2009 Dec 11.
Stable isotope probing (SIP) was used to identify the aerobic toluene-degrading microorganism in soil microcosms. Several approaches (terminal restriction fragment length polymorphism, 16S rRNA gene sequencing, and quantitative PCR) provided evidence that the microorganism responsible was a member of the genus Polaromonas and could grow on toluene. This microorganism also transformed benzene, but not m-xylene or cis-dichloroethene.
稳定同位素探测(SIP)被用于鉴定土壤微宇宙中的好氧甲苯降解微生物。几种方法(末端限制性片段长度多态性、16S rRNA 基因测序和定量 PCR)提供的证据表明,负责的微生物是极地单胞菌属的一个成员,能够以甲苯为生长基质。这种微生物还能转化苯,但不能转化间二甲苯或顺式二氯乙烯。