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污染场地微宇宙中甲苯的降解与 Polaromonas 菌株之间的直接联系。

Direct link between toluene degradation in contaminated-site microcosms and a Polaromonas strain.

机构信息

Department of Civil and Environmental Engineering, Michigan State University, East Lansing, Michigan 48824, USA.

出版信息

Appl Environ Microbiol. 2010 Feb;76(3):956-9. doi: 10.1128/AEM.01364-09. Epub 2009 Dec 11.

DOI:10.1128/AEM.01364-09
PMID:20008173
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2813006/
Abstract

Stable isotope probing (SIP) was used to identify the aerobic toluene-degrading microorganism in soil microcosms. Several approaches (terminal restriction fragment length polymorphism, 16S rRNA gene sequencing, and quantitative PCR) provided evidence that the microorganism responsible was a member of the genus Polaromonas and could grow on toluene. This microorganism also transformed benzene, but not m-xylene or cis-dichloroethene.

摘要

稳定同位素探测（SIP）被用于鉴定土壤微宇宙中的好氧甲苯降解微生物。几种方法（末端限制性片段长度多态性、16S rRNA 基因测序和定量 PCR）提供的证据表明，负责的微生物是极地单胞菌属的一个成员，能够以甲苯为生长基质。这种微生物还能转化苯，但不能转化间二甲苯或顺式二氯乙烯。

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