Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
Phys Med Biol. 2010 Jan 7;55(1):191-206. doi: 10.1088/0031-9155/55/1/011.
Optical coherence tomography (OCT) provides high-resolution, cross-sectional imaging of tissue microstructure in situ and in real time, while fluorescence molecular imaging (FMI) enables the visualization of basic molecular processes. There is a great deal of interest in combining these two modalities so that the tissue's structural and molecular information can be obtained simultaneously. This could greatly benefit biomedical applications such as detecting early diseases and monitoring therapeutic interventions. In this research, an optical system that combines OCT and FMI was developed. The system demonstrated that it could co-register en face OCT and FMI images with a 2.4 x 2.4 mm(2) field-of-view. The transverse resolutions of OCT and FMI of the system are both approximately 10 microm. Capillary tubes filled with fluorescent dye Cy 5.5 in different concentrations under a scattering medium are used as the phantom. En face OCT images of the phantoms were obtained and successfully co-registered with FMI images that were acquired simultaneously. A linear relationship between FMI intensity and dye concentration was observed. The relationship between FMI intensity and target fluorescence tube depth measured by OCT images was also observed and compared with theoretical modeling. This relationship could help in correcting reconstructed dye concentration. Imaging of colon polyps of the APC(min) mouse model is presented as an example of biological applications of this co-registered OCT/FMI system.
光学相干断层扫描（OCT）提供组织微观结构的高分辨率、横截面成像，实时原位，而荧光分子成像（FMI）能够可视化基本的分子过程。人们对将这两种模式结合起来非常感兴趣，以便同时获得组织的结构和分子信息。这将极大地有益于生物医学应用，如早期疾病检测和治疗干预监测。在这项研究中，开发了一种结合 OCT 和 FMI 的光学系统。该系统证明它可以以 2.4 x 2.4 毫米（2）的视场共注册 OCT 和 FMI 图像。该系统的 OCT 和 FMI 的横向分辨率均约为 10 微米。在散射介质下，使用充满不同浓度荧光染料 Cy 5.5 的毛细管作为模型。获得了模型的 OCT 图像，并与同时获得的 FMI 图像成功地共注册。观察到 FMI 强度与染料浓度之间存在线性关系。还观察到并比较了 OCT 图像测量的 FMI 强度与目标荧光管深度之间的关系，并与理论建模进行了比较。这种关系有助于校正重建的染料浓度。以 APC(min) 小鼠模型的结肠息肉成像为例，展示了这种共注册的 OCT/FMI 系统的生物应用。
