用于监测体外人类心脏类器官跳动的双模式成像系统。
Dual-modality imaging system for monitoring human heart organoids beating in vitro.
出版信息
Opt Lett. 2023 Aug 1;48(15):3929-3932. doi: 10.1364/OL.493824.
Abstract
To reveal the three-dimensional microstructure and calcium dynamics of human heart organoids (hHOs), we developed a dual-modality imaging system combining the advantages of optical coherence tomography (OCT) and fluorescence microscopy. OCT provides high-resolution volumetric structural information, while fluorescence imaging indicates the electrophysiology of the hHOs' beating behavior. We verified that concurrent OCT motion mode (M-mode) and calcium imaging retrieved the same beating pattern from the heart organoids. We further applied dynamic contrast OCT (DyC-OCT) analysis to strengthen the verification and localize the beating clusters inside the hHOs. This imaging platform provides a powerful tool for studying and assessing hHOs in vitro, with potential applications in disease modeling and drug screening.
摘要
为了揭示人类心脏类器官（hHOs）的三维微观结构和钙动力学，我们开发了一种结合光学相干断层扫描（OCT）和荧光显微镜优点的双模式成像系统。OCT 提供高分辨率的体积结构信息，而荧光成像则指示 hHOs 跳动行为的电生理学。我们验证了同时的 OCT 运动模式（M 模式）和钙成像从心脏类器官中检索到相同的跳动模式。我们进一步应用动态对比 OCT（DyC-OCT）分析来加强验证并定位 hHOs 内的跳动簇。该成像平台为研究和评估体外 hHOs 提供了有力工具，具有在疾病建模和药物筛选中的潜在应用。
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