离心运动后人类肌肉中白细胞积累的时程。

Time course of leukocyte accumulation in human muscle after eccentric exercise.

机构信息

Norwegian School of Sport Sciences, Oslo, Norway.

出版信息

Med Sci Sports Exerc. 2010 Jan;42(1):75-85. doi: 10.1249/MSS.0b013e3181ac7adb.

DOI:10.1249/MSS.0b013e3181ac7adb

PMID:20010127

Abstract

PURPOSE

To investigate the time course of leukocyte accumulation in eccentric exercised human muscles and its relation to recovery of muscle function and soreness.

METHODS

Eleven young males performed 300 unilateral, maximal voluntary, eccentric actions with the musculus quadriceps femoris (30 degrees x s(-1)). Before and at regular intervals for 7 d after exercise, force-generating capacity was measured with maximal concentric knee extensions (60 degrees x s(-1)). Accumulation of radiolabeled (autologous) leukocytes was measured with scintigraphy. Biopsies from musculus vastus lateralis were obtained 0.5, 4, 8, 24, 96, and 168 h after exercise from both the exercised leg and the control leg. Muscle cross-sections were stained with antibodies against leukocytes (CD16 and CD68). Muscle soreness was rated on a visual analog scale.

RESULTS

Immediately after exercise, the subjects' ability to generate force was reduced by 47 +/- 5%. Muscle function recovered slowly and was not fully restored after 1 wk. Radiolabeled leukocytes accumulated in the muscles during the first hour (3-24 h) after exercise, and leukocytes were at the same time observed histologically, primarily in the endomysium and perimysium. A part of the accumulated radiolabeled leukocytes appeared to be located within local blood vessels. The highest numbers of CD16(+) and CD68(+) cells were found 4 and 7 d after exercise. There was a positive correlation between accumulation of radiolabeled leukocytes and muscle weakness measured 1-3 d after exercise (r = 0.8, P < 0.05) and, surprisingly, a negative correlation between radiolabeled leukocyte accumulation and muscle soreness (r = -0.96, P < 0.01).

CONCLUSION

Exercise-induced muscle damage initiated a rapid local inflammatory response that gradually increased over the next days. Halted recovery of muscle function was associated with local accumulation of leukocytes, whereas muscle soreness could not be explained by the presence of leukocytes.

摘要

目的

研究白细胞在离心运动后人类肌肉中的积累时间过程及其与肌肉功能和酸痛恢复的关系。

方法

11 名年轻男性进行了 300 次单侧、最大自主、离心运动，运动肌肉为股四头肌（30 度/s）。在运动前和运动后第 7 天的定期时间间隔内，通过最大向心膝关节伸展（60 度/s）测量产生力的能力。通过闪烁扫描术测量放射性标记的（自体）白细胞的积累。在运动后 0.5、4、8、24、96 和 168 小时，从运动腿和对照腿获取股外侧肌的活检标本。用针对白细胞（CD16 和 CD68）的抗体对肌肉切片进行染色。肌肉酸痛通过视觉模拟量表进行评分。

结果

运动后立即，受试者的肌力减少了 47±5%。肌肉功能恢复缓慢，1 周后仍未完全恢复。放射性标记的白细胞在运动后 1 小时内（3-24 小时）在肌肉中积累，并且在组织学上同时观察到白细胞，主要位于肌内膜和肌束膜内。一部分积累的放射性标记的白细胞似乎位于局部血管内。CD16(+)和 CD68(+)细胞数量最多分别出现在运动后 4 天和 7 天。在运动后 1-3 天测量的放射性标记白细胞的积累与肌肉无力之间存在正相关（r=0.8，P<0.05），令人惊讶的是，放射性标记白细胞的积累与肌肉酸痛之间存在负相关（r=-0.96，P<0.01）。