Department of Dermatovenereology, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
Rheumatol Int. 2011 Mar;31(3):321-6. doi: 10.1007/s00296-009-1254-3. Epub 2009 Dec 16.
The aim of this study is to investigate the levels of heme oxygenase-1 (HO-1), insulin-like growth factor-1 (IGF-1) and oxidative stress parameters including malondialdehyde (MDA), total antioxidant capacity (T-AOC) and the activities of total superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), catalase (CAT) for exploring the correlations between these biological indexes and the clinical manifestations in Henoch-Schonlein purpura (HSP) patients. 36 patients with different phases of HSP and 16 age- and sex-matched controls were enrolled. MDA level, T-AOC and the activities of SOD, GSH-PX and CAT were measured by spectrophotometry. HO-1 and IGF-1 levels were detected by enzyme-linked immunosorbent assay. Significant higher MDA level, lower T-AOC, SOD, GSH-PX activities were shown in active phase of HSP, respectively, compared with those in early resolution phase of HSP (p < 0.001, <0.001, 0.017, <0.001, respectively) and the control subjects (p < 0.001, <0.001, 0.01, 0.008, respectively). HO-1 (both p < 0.001) and IGF-1 (p < 0.001, 0.009, respectively) levels in active phase and early resolution phase of HSP were significantly higher than those in normal controls. The changes of HO-1 and IGF-1 levels were coincident with overall clinical scores (r = 0.71, p < 0.001; r = 0.615, p < 0.001, respectively). The HO-1 level was found as positive correlation with MDA levels (r = 0.395, p = 0.017), but negative correlations with T-AOC (r = -0.409, p = 0.013) and SOD activities (r = -0.352, p = 0.035). HO-1 and IGF-1 were possibly involved in the pathogenesis of HSP; they could be the marker for evaluating the severity of the disease.
本研究旨在探讨血红素加氧酶-1（HO-1）、胰岛素样生长因子-1（IGF-1）和氧化应激参数（包括丙二醛（MDA）、总抗氧化能力（T-AOC）以及总超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）、过氧化氢酶（CAT）的活性）水平，以探讨这些生物指标与过敏性紫癜（HSP）患者临床表现之间的相关性。共纳入 36 例处于不同阶段的 HSP 患者和 16 名年龄和性别匹配的对照者。采用分光光度法测定 MDA 水平、T-AOC 及 SOD、GSH-PX 和 CAT 的活性。采用酶联免疫吸附试验检测 HO-1 和 IGF-1 水平。与 HSP 早期缓解期（p<0.001、<0.001、0.017、<0.001，分别）和对照组（p<0.001、<0.001、0.01、0.008，分别）相比，HSP 活动期的 MDA 水平更高，T-AOC、SOD、GSH-PX 活性均较低（均为 p<0.001）。HSP 活动期和早期缓解期的 HO-1（均为 p<0.001）和 IGF-1（p<0.001、0.009，分别）水平明显高于正常对照组。HO-1 和 IGF-1 水平的变化与总临床评分一致（r=0.71，p<0.001；r=0.615，p<0.001，分别）。HO-1 水平与 MDA 水平呈正相关（r=0.395，p=0.017），与 T-AOC 呈负相关（r=-0.409，p=0.013），与 SOD 活性呈负相关（r=-0.352，p=0.035）。HO-1 和 IGF-1 可能参与 HSP 的发病机制；它们可以作为评估疾病严重程度的标志物。
