Department of Nanomedicine and BioMedical Engineering, University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
Proteomics. 2010 Feb;10(3):496-505. doi: 10.1002/pmic.200900346.
The advanced properties of mesoporous silica have been demonstrated in applications, which include chemical sensing, filtration, catalysis, drug delivery and selective biomolecular uptake. These properties depend on the architectural, physical and chemical properties of the material, which in turn are determined by the processing parameters in evaporation-induced self-assembly. In this study, we introduce a combinatorial approach for the removal of the high molecular weight proteins and for the specific isolation and enrichment of low molecular weight species. This approach is based on mesoporous silica chips able to fractionate, selectively harvest and protect from enzymatic degradation, peptides and proteins present in complex human biological fluids. We present the characterization of the harvesting properties of a wide range of mesoporous chips using a library of peptides and proteins standard and their selectivity on the recovery of serum peptidome. Using MALDI-TOF-MS, we established the correlation between the harvesting specificity and the physicochemical properties of mesoporous silica surfaces. The introduction of this mesoporous material with fine controlled properties will provide a powerful platform for proteomics application offering a rapid and efficient methodology for low molecular weight biomarker discovery.
介孔硅的先进特性在多个领域得到了证明，包括化学传感、过滤、催化、药物输送和选择性生物分子摄取。这些特性取决于材料的结构、物理和化学特性，而这些特性又取决于蒸发诱导自组装过程中的处理参数。在这项研究中，我们引入了一种组合方法，用于去除高分子量蛋白质，并对低分子量物质进行特异性分离和富集。这种方法基于能够对复杂的人体生物流体中的肽和蛋白质进行分离、选择性收获和保护免受酶降解的介孔硅芯片。我们使用肽和蛋白质标准文库对一系列介孔芯片的收获特性进行了表征，并研究了它们对血清肽组回收的选择性。我们使用 MALDI-TOF-MS 建立了收获特异性与介孔硅表面物理化学性质之间的相关性。这种具有精细控制特性的介孔材料的引入将为蛋白质组学应用提供一个强大的平台，为低分子量生物标志物的发现提供快速有效的方法。
