Laser Biomedical Applications and Instrumentation Division, Raja Ramanna Centre for Advanced Technology, Indore, India. sunita_agni @ yahoo.co.in
Skin Pharmacol Physiol. 2010;23(2):79-85. doi: 10.1159/000265678. Epub 2009 Dec 14.
We report the results of a study carried out to investigate the effect of helium-neon (He-Ne) laser (632.8 nm) irradiation on the hair follicle growth cycle of testosterone-treated and untreated mice. Both histology and optical coherence tomography (OCT) were used for the measurement of hair follicle length and the relative percentage of hair follicles in different growth phases. A positive correlation (R = 0.96) was observed for the lengths of hair follicles measured by both methods. Further, the ratios of the lengths of hair follicles in the anagen and catagen phases obtained by both methods were nearly the same. However, the length of the hair follicles measured by both methods differed by a factor of 1.6, with histology showing smaller lengths. He-Ne laser irradiation (at approximately 1 J/cm(2)) of the skin of both the control and the testosterone-treated mice was observed to lead to a significant increase (p < 0.05) in % anagen, indicating stimulation of hair growth. The study also demonstrates that OCT can be used to monitor the hair follicle growth cycle, and thus hair follicle disorders or treatment efficacy during alopecia.
我们报告了一项研究的结果，该研究旨在调查氦氖（He-Ne）激光（632.8nm）照射对经和未经睾酮处理的小鼠毛囊生长周期的影响。组织学和光学相干断层扫描（OCT）都用于测量毛囊长度和不同生长阶段的毛囊相对百分比。两种方法测量的毛囊长度呈正相关（R=0.96）。此外，两种方法获得的生长期和退行期毛囊长度之比几乎相同。然而，两种方法测量的毛囊长度相差 1.6 倍，组织学显示的长度较小。观察到对对照组和睾酮处理组小鼠皮肤进行 He-Ne 激光照射（约 1J/cm(2)）会导致生长期百分比显著增加（p<0.05），表明刺激毛发生长。该研究还表明，OCT 可用于监测毛囊生长周期，从而监测脱发期间的毛囊疾病或治疗效果。
