Hugo E R, McLaughlin W R, Oh K H, Tuovinen O H
Department of Molecular Genetics, Ohio State University, Columbus 43210-1292.
Invest Ophthalmol Vis Sci. 1991 Mar;32(3):655-7.
A simple, quantitative plate assay has been developed for use in the enumeration of Acanthamoeba. The technique uses an agarose overlay and low-nutrient medium to support the growth of amoebae on a bacterial lawn. The authors found that in this assay, individual trophozoites or dormant cysts will cause plaques to form in an Enterobacter aerogenes lawn. With the assay, it is possible to quantitatively assess the effects of various disinfectant compounds on the viability of Acanthamoeba. The authors used this assay to enumerate Acanthamoeba cyst viability after chemical disinfection in contact lens care solutions. The inactivation data indicated major differences among the four test solutions evaluated.
已开发出一种简单的定量平板测定法用于棘阿米巴的计数。该技术使用琼脂糖覆盖层和低营养培养基来支持变形虫在细菌菌苔上生长。作者发现，在此测定法中，单个滋养体或休眠包囊会在产气肠杆菌菌苔中形成噬菌斑。通过该测定法，可以定量评估各种消毒剂化合物对棘阿米巴生存能力的影响。作者使用此测定法来计数隐形眼镜护理液化学消毒后棘阿米巴包囊的生存能力。灭活数据表明所评估的四种测试溶液之间存在重大差异。
