Shoff Megan, Rogerson Andrew, Schatz Scott, Seal David
Oceanographic Center, Nova Southeastern University, Dania Beach, Florida, USA.
Optom Vis Sci. 2007 Mar;84(3):202-7. doi: 10.1097/OPX.0b013e3180339f81.
A novel method has been developed to compare Acanthamoeba strain differences by testing their susceptibility to multipurpose contact lens cleaning solutions (MPS).
This method uses surface-attached amoebae that mimic cells attached to a contact lens. In brief, acanthamoebae were grown on non-nutrient agar plates with Escherichia coli prey. Blocks of agar with cysts or trophozoites (approximately 50 cells) were cut out and transferred to MPS [ReNu MultiPlus; (Bausch & Lomb, Rochester, NY), Complete (Allergan, Irvine, CA), and Opti-Free Express (Alcon, Ft. Worth, TX)] for up to 24 h treatment. After neutralizing in Dey Engley broth (Difco), blocks were washed in amoeba saline and reinoculated onto fresh prey bacteria. Positive growth was indicative of survival.
Testing showed that the efficacy of the three MPS was different. Opti-Free was the most effective against cysts, followed by ReNu while Complete was relatively ineffective. Not surprisingly, trophozoites were more susceptible than cysts.
Findings for individual MPS complement previous work with other methods. This study has also identified that environmental strains vary in their sensitivity to disinfecting solutions. Overall, Acanthamoeba genotypes T3, T5, and T11 from the environment were more resistant than the T4 isolates from the cornea and beach. This resilience supports previous work on temperature tolerance, in which T3 and T5, and T11 acanthamoebae grew in temperatures up to 41 degrees C. Investigators should therefore consider the strain genotype and its source before embarking on MPS sensitivity testing.
已开发出一种新方法，通过测试棘阿米巴菌株对多功能隐形眼镜护理液（MPS）的敏感性来比较其菌株差异。
该方法使用模拟附着在隐形眼镜上的细胞的表面附着变形虫。简而言之，棘阿米巴在含有大肠杆菌猎物的无营养琼脂平板上生长。切出含有包囊或滋养体（约50个细胞）的琼脂块，并转移至MPS [ReNu MultiPlus；（博士伦公司，纽约州罗切斯特），Complete（爱尔康公司，加利福尼亚州欧文），以及Opti-Free Express（爱尔康公司，得克萨斯州沃思堡）] 中进行长达24小时的处理。在迪伊 - 恩格利肉汤（Difco）中中和后，将琼脂块在阿米巴盐水中洗涤，并重新接种到新鲜的猎物细菌上。阳性生长表明存活。
测试表明三种MPS的功效不同。Opti-Free对包囊最有效，其次是ReNu，而Complete相对无效。不出所料，滋养体比包囊更易受影响。
个别MPS的研究结果补充了先前其他方法的研究工作。本研究还确定环境菌株对消毒溶液的敏感性各不相同。总体而言，来自环境的棘阿米巴基因型T3、T5和T11比来自角膜和海滩的T4分离株更具抗性。这种抗性支持了先前关于温度耐受性的研究工作，其中T3、T5和T11棘阿米巴能在高达41摄氏度的温度下生长。因此，研究人员在进行MPS敏感性测试之前应考虑菌株基因型及其来源。
