Department of Materials Science, Friedrich-Alexander-University of Erlangen-Nuremberg, Germany.
Integr Biol (Camb). 2009 Sep;1(8-9):525-32. doi: 10.1039/b908196h. Epub 2009 Jun 19.
This work reports on the behavior of mesenchymal stem cells on anodic ZrO(2) nanotube layers grown by a self-ordering process on zirconium with defined diameters between 10 and 50 nm. It is demonstrated that mesenchymal stem cells show a size-specific reaction to these nanoscale patterned surfaces. We compare the behavior on these ZrO(2) nanotubes to findings on TiO(2) nanotubes of different diameters. For both nanotube materials, TiO(2) and ZrO(2), cell adhesion and spreading are enhanced for nanotube diameters of approximately 15-30 nm, while a strong decay in cell activity is observed for diameters >50 nm. Focal complex formation on adherent cells is selectively modulated by the specific nanoscale. Moreover, even if the surface chemistry of the nanotubes is completely modified with a dense AuPd coating onto the formed nanotube layers, or the length of the nanotubes is varied, the observed nano size effects still prevail. This demonstrates how strong the pure geometric diameter dependence in the range between 15 and 100 nm dominates over other possible effects on cell activity.
这项工作报道了在具有 10 至 50nm 之间定义直径的锆上通过自组织过程生长的阳极氧化 ZrO2 纳米管层上的间充质干细胞的行为。结果表明，间充质干细胞对这些纳米级图案化表面表现出尺寸特异性反应。我们将这些 ZrO2 纳米管上的行为与不同直径的 TiO2 纳米管上的发现进行了比较。对于这两种纳米管材料 TiO2 和 ZrO2，纳米管直径约为 15-30nm 时，细胞黏附和铺展增强，而直径大于 50nm 时，细胞活性强烈下降。在附着的细胞上，粘着斑的形成被特定的纳米尺度选择性地调节。此外，即使纳米管的表面化学性质完全被形成的纳米管层上的密集 AuPd 涂层修饰，或者纳米管的长度发生变化，观察到的纳米尺寸效应仍然占主导地位。这证明了在 15 至 100nm 范围内，纯几何直径依赖性是多么强烈地支配着细胞活性的其他可能影响。
