Bunescu Andrei, Garric Jeanne, Vollat Bernard, Canet-Soulas Emmanuelle, Graveron-Demilly Danielle, Fauvelle Florence
Cemagref, UR MALY, Laboratory of Ecotoxicology, 3 bis quai Chauveau, Lyon, France.
Mol Biosyst. 2010 Jan;6(1):121-5. doi: 10.1039/b915417e. Epub 2009 Sep 29.
The method concerning in vivo proton HR-MAS NMR metabolic profiling of the freshwater cladoceran Daphnia magna is presented. Viability tests of D. magna under different spinning rates were performed. All surviving daphnids after analysis have developed eggs and embryos like control animals. Better survival rate at the slowest rotation speed were observed. The maximum length of analysis during which the integrity of the daphnid is maintained was assessed. The recorded proton spectra of in vivo daphnia were attributed to lipids from the triglycerol category. Saturated and unsaturated omega-3 like fatty acid moieties of triacylglycerol were well identified. The relationship between physiological state of daphnids and lipid profile are discussed.
本文介绍了关于淡水枝角类大型溞体内质子高分辨魔角旋转核磁共振代谢谱分析的方法。对不同旋转速率下大型溞的活力进行了测试。分析后所有存活的溞都像对照动物一样发育出了卵和胚胎。观察到在最慢旋转速度下有更高的存活率。评估了在保持溞完整性的情况下最长的分析时长。所记录的大型溞体内质子谱归属于甘油三酯类脂质。很好地鉴定出了三酰甘油的饱和及不饱和ω-3类脂肪酸部分。讨论了大型溞的生理状态与脂质谱之间的关系。
