Department of Surgery, University of Vermont, Burlington, Vermont 05401, USA.
J Surg Res. 2010 Jun 1;161(1):18-22. doi: 10.1016/j.jss.2009.06.027. Epub 2009 Jul 21.
Lung cancer remains the leading cause of cancer mortality in the United States. Resveratrol is a potent antioxidant found in grapes that inhibits several types of cancer, including lung cancer. Herein, we investigated the effects of pterostilbene, an analog of resveratrol found in blueberries, on lung cancer, in vitro. We hypothesized that pterostilbene would inhibit lung cancer cell growth in vitro by a pro-apoptotic mechanism.
Two lung cancer cell lines (NCI-H460 and SK-MES-1) were cultured using standard techniques. Cells were treated with increasing doses of pterostilbene (10-100 microM). Cell viability was measured at 24, 48, and 72h using a MTT assay. Apo-ONE Caspase-3/7 assay was used to evaluate caspase activity. T-test and two-way ANOVA were used for statistical analysis.
Pterostilbene significantly decreased cell viability in lung cancer cells in a concentration- and time-dependent manner (P<0.001). Concentrations greater than 20 microM of pterostilbene produced significant growth inhibition by 72h (P<0.001). Apoptosis and caspase-3/7 activity were significantly increased by pterostilbene treatment (P<0.05).
Pterostilbene inhibits growth via apoptosis induction in vitro. Further in vitro mechanistic studies and in vivo experiments are warranted to determine the potential role for pterostilbene in lung cancer treatment or prevention.
肺癌仍然是美国癌症死亡的主要原因。白藜芦醇是一种存在于葡萄中的有效抗氧化剂，可抑制多种癌症，包括肺癌。在此，我们研究了存在于蓝莓中的白藜芦醇类似物紫檀芪对肺癌的体外作用。我们假设紫檀芪通过促进细胞凋亡的机制抑制体外肺癌细胞的生长。
采用标准技术培养两种肺癌细胞系（NCI-H460 和 SK-MES-1）。用不同浓度的紫檀芪（10-100 μM）处理细胞。采用 MTT 法在 24、48 和 72h 时测定细胞活力。采用 Apo-ONE Caspase-3/7 测定法评估半胱天冬酶活性。采用 t 检验和双因素方差分析进行统计学分析。
紫檀芪呈浓度和时间依赖性显著降低肺癌细胞活力（P<0.001）。紫檀芪浓度大于 20 μM 时，72h 时生长抑制显著（P<0.001）。紫檀芪处理后细胞凋亡和半胱天冬酶-3/7 活性显著增加（P<0.05）。
紫檀芪通过诱导细胞凋亡抑制体外生长。需要进一步的体外机制研究和体内实验来确定紫檀芪在肺癌治疗或预防中的潜在作用。
