University of Vermont/Fletcher Allen Health Care, Burlington, VT, USA.
J Gastrointest Surg. 2010 May;14(5):873-9. doi: 10.1007/s11605-010-1164-4. Epub 2010 Feb 6.
Stilbenes are phenolic compounds present in grapes and blueberries. Resveratrol, a naturally occurring compound present in grapes, has been shown to have potent antioxidant properties as well as an ability to induce apoptosis. Resveratrol has also been reported to have significant inhibitory effects against a variety of primary tumors including breast, colon, and prostate. Pterostilbene, a naturally occurring analogue of resveratrol found in blueberries, also has antioxidant and antiproliferative properties. It is also substantially more bioavailable orally than resveratrol. These effects have not been studied in pancreatic cancer. We hypothesized that pterostilbene would inhibit pancreatic cancer cell growth in vitro.
Two pancreatic cancer cell lines (MIA PaCa and PANC-1) were cultured using standard techniques. Cells were treated with graduated doses of pterostilbene ranging from 10 to 100 microM. Cell viability was measured by MTT at 24, 48, and 72 h.
Pterostilbene decreases cell viability in both cancer cell lines in a concentration- and time-dependent manner. Higher doses (75-100 microM) caused a significant reduction in cell viability at 24 and 48 h. However, by 72 h, all tested concentrations of pterostilbene (10 to 100 microM) resulted in significantly reduced cell viability in both pancreatic cancer cell lines in a dose-dependent fashion. Pterostilbene caused a dose-dependent 10-63% inhibition in MIA PaCa-2 cells and 10-75% inhibition in PANC-1 cells.
Treatment of pancreatic cancer cells in vitro with Pterostilbene leads to inhibition of cell proliferation and/or cell death, cell cycle arrrest, mitochondrial membrane depolarization, and activation of effector caspases. This naturally occurring agent may have a role in treating pancreatic cancer.
Pterostilbene inhibits the growth of pancreatic cancer in vitro. Further, in vitro mechanistic studies and in vivo experiments are warranted to determine its potential for the treatment of pancreatic cancer.
白藜芦醇是存在于葡萄和蓝莓中的酚类化合物。白藜芦醇是葡萄中天然存在的一种化合物，具有很强的抗氧化特性，并且能够诱导细胞凋亡。白藜芦醇还被报道对多种原发性肿瘤具有显著的抑制作用，包括乳腺癌、结肠癌和前列腺癌。二甲氧基二苯乙烯是蓝莓中白藜芦醇的一种天然类似物，也具有抗氧化和抗增殖作用。它的口服生物利用度也比白藜芦醇高得多。这些作用尚未在胰腺癌中进行研究。我们假设二甲氧基二苯乙烯会抑制胰腺癌细胞在体外的生长。
使用标准技术培养两种胰腺癌细胞系（MIA PaCa 和 PANC-1）。用从 10 到 100 μM 的梯度剂量的二甲氧基二苯乙烯处理细胞。在 24、48 和 72 h 通过 MTT 测量细胞活力。
二甲氧基二苯乙烯以浓度和时间依赖的方式降低两种癌细胞系中的细胞活力。较高剂量（75-100 μM）在 24 和 48 h 时导致细胞活力显著降低。然而，在 72 h 时，二甲氧基二苯乙烯的所有测试浓度（10 至 100 μM）均导致两种胰腺癌细胞系中的细胞活力呈剂量依赖性显著降低。二甲氧基二苯乙烯导致 MIA PaCa-2 细胞的 10-63%抑制和 PANC-1 细胞的 10-75%抑制。
体外用二甲氧基二苯乙烯处理胰腺癌细胞导致细胞增殖和/或细胞死亡、细胞周期阻滞、线粒体膜去极化和效应半胱天冬酶的激活。这种天然存在的物质可能在治疗胰腺癌中发挥作用。
二甲氧基二苯乙烯抑制胰腺癌细胞在体外的生长。进一步的，需要进行体外机制研究和体内实验，以确定其治疗胰腺癌的潜力。
