Department of Pharmacology, Faculty of Pharmacy, Hamdard University, New Delhi, India.
Indian J Pharmacol. 2008 Oct;40(5):215-20. doi: 10.4103/0253-7613.44153.
The present study was carried out to evaluate the neuroprotective effect of the aqueous extract of Embelia ribes, in focal ischemic brain.
Adult male Wistar albino rats were fed with the aqueous extract of Embelia ribes (100 and 200 mg/kg, p.o.) for 30 days. After 30 days of feeding, all the animals were anaesthetized with chloral hydrate (400 mg/kg, i.p.). The right middle cerebral artery was occluded with a 4-0 suture for 2 h. The suture was removed after 2 h, to allow reperfusion injury. The animals were used for grip strength measurement, biochemical estimation in serum and brain tissue (hippocampus and frontal cortex) and cerebral infarct size measurement.
In the ischemic group, a significant (P < 0.01) alteration in the markers of oxidative damage (thiobarbituric acid reactive substances (TBARS); reduced glutathione (GSH); glutathione peroxidase (GPx); glutathione reductase (GR); and, glutathione-S-transferase (GST)) was observed in the hippocampus and frontal cortex, as compared to sham operated rats. We observed that the animals treated with the aqueous extract of Embelia ribes had a significant (P < 0.01) increase in the poststroke grip strength activity. Further, supplementation with aqueous extract of Embelia ribes reversed the levels/activities of the above mentioned biochemical parameters significantly (P< 0.01) and also resulted in decreased cerebral infarct area, as compared to the ischemic group.
The results of our study, for the first time, provide clear evidence that aqueous extract of Embelia ribes pretreatment ameliorates cerebral ischemia/reperfusion injury and enhances the antioxidant defense against middle cerebral artery occlusion-induced cerebral infarction in rats; it exhibits neuroprotective property.
本研究旨在评估山茱萸水提物对局灶性缺血性脑损伤的神经保护作用。
成年雄性 Wistar 白化大鼠用山茱萸水提物（100 和 200mg/kg，po）喂养 30 天。喂养 30 天后，所有动物均用氯醛（400mg/kg，ip）麻醉。用 4-0 缝线将右侧大脑中动脉闭塞 2 小时以造成缺血再灌注损伤。2 小时后取出缝线以允许再灌注损伤。用握力计测量动物的握力，检测血清和脑组织（海马和前额皮质）中的生化指标，并测量脑梗死面积。
与假手术组大鼠相比，缺血组大鼠海马和前额皮质的氧化损伤标志物（丙二醛（TBARS）；还原型谷胱甘肽（GSH）；谷胱甘肽过氧化物酶（GPx）；谷胱甘肽还原酶（GR）；谷胱甘肽-S-转移酶（GST））明显改变（P<0.01）。我们观察到，用山茱萸水提物处理的动物在脑卒中后握力活动中明显增加（P<0.01）。此外，与缺血组相比，山茱萸水提物的补充显著逆转了上述生化参数的水平/活性（P<0.01），并减少了脑梗死面积。
我们的研究结果首次提供了明确的证据，表明山茱萸水提物预处理可改善脑缺血/再灌注损伤，并增强抗氧化防御，防止大脑中动脉闭塞引起的大鼠脑梗死；它表现出神经保护特性。
