使用固定化天然抗原，在患有胶原诱导性关节炎的恒河猴（猕猴）血液淋巴细胞培养物中诱导产生II型胶原特异性抗体。
Induction of type II collagen-specific antibody production in blood lymphocyte cultures of rhesus monkeys (Macaca mulatta) with collagen-induced arthritis using the immobilized native antigen.
作者信息
't Hart B A, Botman C A, Bakker N P
机构信息
Department of Chronic and Infectious Diseases, ITRI-TNO Institute, Rijswijk, The Netherlands.
出版信息
Clin Exp Immunol. 1991 Mar;83(3):375-8. doi: 10.1111/j.1365-2249.1991.tb05646.x.
Abstract
Peripheral blood mononuclear cells (PBMC) from Rhesus monkeys previously immunized with bovine type II collagen to induce arthritis were cultured with the same antigen. Because the native protein is poorly soluble in culture medium a heating step is often used. The antigen in this form induced PBMC proliferation, but epitopes for the induction of antibody production and arthritis were lost. To keep the native protein intact it was coated on affigel beads. With the immobilized antigen specific antibody production could be induced.
摘要
将先前用牛II型胶原免疫以诱导关节炎的恒河猴的外周血单个核细胞（PBMC）与相同抗原一起培养。由于天然蛋白质在培养基中的溶解性较差，因此经常采用加热步骤。这种形式的抗原可诱导PBMC增殖，但诱导抗体产生和关节炎的表位却丧失了。为保持天然蛋白质的完整性，将其包被在Affigel珠上。使用固定化抗原则可诱导特异性抗体产生。
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