离心逆流色谱的新型设计：I. 曲折环形柱
Novel Design for Centrifugal Countercurrent Chromatography: I. Zigzag Toroidal Column.
作者信息
Yang Yi, Aisa Haji Akber, Ito Yoichiro
机构信息
Bioseparation Technology Laboratory, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, 10 Center Drive, Building 10, Room 8N230, Bethesda, MD 20892, USA.
出版信息
J Liq Chromatogr Relat Technol. 2009 Jan 1;32(14):2030-2042. doi: 10.1080/10826070903126856.
Abstract
The toroidal coil using an equilateral triangular core has improved both retention of the stationary phase and peak resolution of the conventional toroidal coil in centrifugal countercurrent chromatography. To further improve the retention of stationary phase and peak resolution, a novel zigzag toroidal coil was designed and the performance of the system was evaluated at various flow rates. The results indicated that both retention of stationary phase and peak resolution were improved as the flow rate was decreased. Modification of the tubing by pressing at given intervals with a pair of pliers improved the peak resolution without increasing the column pressure. All these separations were performed under low column pressure indicating the separation can be further improved by increasing the column length and/or revolution speed without damaging the separation column.
摘要
在离心逆流色谱法中，使用等边三角形芯的环形线圈提高了固定相的保留率和传统环形线圈的峰分辨率。为了进一步提高固定相的保留率和峰分辨率，设计了一种新型的之字形环形线圈，并在不同流速下评估了该系统的性能。结果表明，随着流速降低，固定相的保留率和峰分辨率均得到提高。通过用钳子按给定间隔挤压管道来进行改进，在不增加柱压的情况下提高了峰分辨率。所有这些分离都是在低柱压下进行的，这表明通过增加柱长和/或转速可以进一步改善分离效果，而不会损坏分离柱。
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