Yang Yi, Gu Dongyu, Aisa Haji Akber, Ito Yoichiro
Bioseparation Technology Laboratory, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, 10 Center Drive, Building 10, Room 8N230, Bethesda, MD 20892, USA.
J Liq Chromatogr Relat Technol. 2010 Jan;33(16):1542-1549. doi: 10.1080/10826076.2010.503780.
The conventional toroidal coil in centrifugal countercurrent chromatography has a low level of stationary phase retention, since a half of each helical turn is entirely occupied by the mobile phase. In order to cope with this problem, several new column designs including zigzag, saw-tooth and figure-8 patterns have been introduced and their performance was compared in terms of retention of the stationary phase (Sf), peak resolution (Rs), theoretical plate number (N) and column pressures. Overall results of experiments indicate that the figure-8 column yields the highest Rs when the lower phase is used as the mobile phase. Since the column pressure of all these new columns are much lower than that in the traditional toroidal coil column, the separation efficiency can be improved using a long separation column without a risk of column damage by high back pressure.
离心逆流色谱中的传统环形线圈固定相保留水平较低，因为每个螺旋圈的一半完全被流动相占据。为了解决这个问题，人们引入了几种新的柱设计，包括锯齿形、之字形和8字形，并从固定相保留率（Sf）、峰分辨率（Rs）、理论塔板数（N）和柱压等方面对它们的性能进行了比较。实验的总体结果表明，当下相用作流动相时，8字形柱的Rs最高。由于所有这些新柱的柱压都远低于传统环形线圈柱，因此使用长分离柱可以提高分离效率，而不会有因高背压导致柱损坏的风险。
