Yang Yi, Aisa Haji Akber, Ito Yoichiro
Bioseparation Technology Laboratory, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, 10 Center Drive, Building 10, Room 8N230, Bethesda, MD 20892, USA.
J Liq Chromatogr Relat Technol. 2010 Jan;33(3):336-348. doi: 10.1080/10826070903524100.
The toroidal column using a zigzag pattern has been improved in both retention of the stationary phase and peak resolution. To further improve the retention of stationary phase and peak resolution, a series of novel geometric designs of tubing (plain, mid-clamping, flattened and flat-twisted tubing) was evaluated their performance in CCC. The results showed that the tubing which was flattened vertically against centrifugal force (vert-flattened tubing) produced the best peak resolution among them. Using vert-flattened tubing a series of experiments was performed to study the effects of column capacity and sample size. The results indicated that a 0.25 ml capacity column is ideal for analysis of small amount samples.
采用之字形图案的环形柱在固定相保留和峰分辨率方面均有改进。为了进一步提高固定相保留和峰分辨率，对一系列新型管材几何设计（普通管材、中间夹紧管材、扁平管材和扁平扭曲管材）在CCC中的性能进行了评估。结果表明，垂直于离心力方向压扁的管材（垂直压扁管材）在这些管材中产生了最佳的峰分辨率。使用垂直压扁管材进行了一系列实验以研究柱容量和样品量的影响。结果表明，0.25 ml容量的柱对于少量样品的分析是理想的。
