Li Chang, Dai Yi, Zhang Shu-Xiang, Duan Ying-Hui, Liu Ming-Li, Chen Liu-Yuan, Yao Xin-Sheng
College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, People's Republic of China; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou 510632, People's Republic of China.
Phytochemistry. 2014 Aug;104:105-13. doi: 10.1016/j.phytochem.2014.04.010. Epub 2014 May 12.
Phytochemical investigation on Forsythia suspensa (Thunb.) Vahl afforded 10 compounds, including quinoid glycosides, lignan glycosides, phenylethanoid glycoside and allylbenzene glycoside together with 13 known ones. Their structures were established based on extensive spectroscopic data analyses, including IR, UV, HRESIMS, 1D NMR and 2D NMR. Absolute configurations were determined by ECD calculation method and chemical degradation. In addition, all compounds were evaluated for their antiviral activity against influenza A (H1N1) virus and several were further evaluated against respiratory syncytial virus (RSV) in vitro. Among them, two previously known compounds showed significant activities against RSV with EC50 values of 3.43 and 6.72 μM.
对连翘进行植物化学研究，得到了10种化合物，包括醌类糖苷、木脂素糖苷、苯乙醇苷和烯丙基苯糖苷，以及13种已知化合物。它们的结构是基于广泛的光谱数据分析确定的，包括红外光谱（IR）、紫外光谱（UV）、高分辨电喷雾电离质谱（HRESIMS）、一维核磁共振（1D NMR）和二维核磁共振（2D NMR）。通过电子圆二色光谱（ECD）计算方法和化学降解确定了绝对构型。此外，评估了所有化合物对甲型流感（H1N1）病毒的抗病毒活性，并对其中几种化合物进一步进行了体外抗呼吸道合胞病毒（RSV）的活性评估。其中，两种已知化合物对RSV显示出显著活性，半数有效浓度（EC50）值分别为3.43和6.72 μM。
