Kalinova Zuzana, Halanova Monika, Cislakova Lydia, Sulinova Zlatana, Jarcuska Pavol
Department of Public Health, Pavol Jozef Safarik University, Faculty of Medicine, Srobarova 2, 041 80 Kosice, Slovak Republic.
Ann Agric Environ Med. 2009;16(2):285-8.
Human granulocytic anaplasmosis (HGA) and Lyme borreliosis (LB) are tick-borne and emerging infectious diseases caused by the Anaplasma phagocytophilum and Borrelia burgdorferi species. In Europe, including Slovakia, the principal vector of both pathogens is the common tick - Ixodes ricinus, in which double infections with these pathogens have been reported. The aim of our study was evidence of IgG antibodies against A. phagocytophilum in blood sera of humans with suspects LB from several Clinics of University Hospitals, and the evaluation of the possibility of B. burgdorferi and A. phagocytophilum co-infection in examined patients. The serological method ELISA was used to detect IgM and IgG antibodies against B. burgdorferi. Anti-A. phagocytophilum IgG antibodies were analyzed by the A. phagocytophilum Indirect Immunofluorescence Antibody (IFA) IgG test. A total of 214 human samples (91 men, 123 women) were obtained from patients living in Kosice town and in villages around Kosice (Eastern Slovakia). IgG antibodies against A. phagocytophilum were detected in 15 cases (6 men, 9 women), which represented 7.0 % positivity. Two cases of the co-infection B. burgdorferi with A. phagocytophilum, which equals 0.93 % of the total number, were found.
人粒细胞无形体病（HGA）和莱姆病（LB）是由嗜吞噬细胞无形体和伯氏疏螺旋体引起的蜱传新发传染病。在包括斯洛伐克在内的欧洲，这两种病原体的主要传播媒介是常见的蜱——蓖麻硬蜱，已有关于这些病原体双重感染的报道。我们研究的目的是在几所大学医院诊所疑似患有莱姆病的患者血清中检测抗嗜吞噬细胞无形体的IgG抗体，并评估受检患者中伯氏疏螺旋体和嗜吞噬细胞无形体共同感染的可能性。采用酶联免疫吸附测定（ELISA）血清学方法检测抗伯氏疏螺旋体的IgM和IgG抗体。通过嗜吞噬细胞无形体间接免疫荧光抗体（IFA）IgG试验分析抗嗜吞噬细胞无形体IgG抗体。共从居住在科希策市及科希策周边村庄（斯洛伐克东部）的患者中获取了214份人类样本（91名男性，123名女性）。15例（6名男性，9名女性）检测到抗嗜吞噬细胞无形体的IgG抗体，阳性率为7.0%。发现2例伯氏疏螺旋体与嗜吞噬细胞无形体共同感染病例，占总数的0.93%。
