Stamm L V, Gherardini F C, Parrish E A, Moomaw C R
Department of Epidemiology, School of Public Health, University of North Carolina, Chapel Hill 27599-7400.
Infect Immun. 1991 Apr;59(4):1572-5. doi: 10.1128/iai.59.4.1572-1575.1991.
We examined the heat shock response of the pathogenic spirochetes Treponema pallidum, Borrelia burgdorferi, and Leptospira interrogans and certain saprophytic spirochetes. Cellular proteins synthesized after shifts to higher temperatures were [35S]methionine labeled and analyzed by gel electrophoresis and fluorography. Only T. pallidum failed to exhibit an obvious heat shock response. GroEL and DnaK homologs were identified in the various species, although these proteins were not thermoinducible in T. pallidum or Treponema denticola. DNA hybridization studies indicate that spirochetal groEL and dnaK genes are highly conserved.
我们检测了致病性螺旋体梅毒螺旋体、伯氏疏螺旋体和问号钩端螺旋体以及某些腐生性螺旋体的热休克反应。转移至较高温度后合成的细胞蛋白用[35S]甲硫氨酸标记，并通过凝胶电泳和荧光自显影进行分析。只有梅毒螺旋体未表现出明显的热休克反应。在不同物种中鉴定出了GroEL和DnaK同源物，尽管这些蛋白在梅毒螺旋体或齿龈螺旋体中不是热诱导性的。DNA杂交研究表明，螺旋体的groEL和dnaK基因高度保守。
