Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang Medical College, Institution of Pathogenic Biology, University of South China, Hengyang, China.
Clinical Laboratory, The Second Affiliated Hospital of University of South China, Hengyang, China.
Front Immunol. 2021 Jan 8;11:577129. doi: 10.3389/fimmu.2020.577129. eCollection 2020.
The spirochetal pathogen causes 5 million new cases of venereal syphilis worldwide each year. One major obstacle to syphilis prevention and treatment is the lack of suitable experimental animal models to study its pathogenesis. Accordingly, in this study, we further evaluated the responses of mice to . Quantitative polymerase chain reaction showed that could colonize the heart, liver, spleen, kidneys, and testicles of C57BL/6 mice, and the organism may be able to rapidly penetrate the blood-brain barrier in mice by 24 h after infection. In subsequent rabbit infectivity tests, we observed evident signs of the microorganism in the mouse lymph node suspension. After infection, bacterial loads were higher in the tissues than in the blood of C57BL/6 mice. Moreover, a significant Th1 immune response was recorded by cytokine assays. Flow cytometric analysis suggested an obvious increase in the proportion of CD3 T and CD4 T cells in the spleen cells in the infected mice. Thus, improving our understanding of the response of C57BL/6 mice for will help to comprehensive elucidate the pathogenic mechanisms of this bacterium and lay the foundation for the development of a new research model of
螺旋体病原体每年在全球导致 500 万例新的性传播梅毒病例。梅毒预防和治疗的一个主要障碍是缺乏合适的实验动物模型来研究其发病机制。因此，在本研究中，我们进一步评估了小鼠对 的反应。定量聚合酶链反应显示， 可以定植于 C57BL/6 小鼠的心脏、肝脏、脾脏、肾脏和睾丸，并且该生物体在感染后 24 小时内可能能够迅速穿透小鼠的血脑屏障。在随后的兔感染性试验中，我们在小鼠淋巴结悬浮液中观察到了明显的微生物迹象。感染后，组织中的细菌负荷高于 C57BL/6 小鼠的血液。此外，细胞因子测定记录到明显的 Th1 免疫反应。流式细胞术分析表明，感染小鼠脾脏细胞中 CD3 T 和 CD4 T 细胞的比例明显增加。因此，提高我们对 C57BL/6 小鼠 反应的理解将有助于全面阐明该细菌的致病机制，并为新的研究模型的开发奠定基础。
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