Carreiro M M, Laux D C, Nelson D R
Department of Microbiology, University of Rhode Island, Kingston 02881.
Infect Immun. 1990 Jul;58(7):2186-91. doi: 10.1128/iai.58.7.2186-2191.1990.
The heat shock response of Borrelia burgdorferi B31 cells was characterized with regard to the heat shock proteins (Hsps) produced. Five to seven Hsps were detected by sodium dodecyl sulfate-gel electrophoresis and fluorography of proteins from cells labeled with [35S]methionine after shifts from 33 degrees C to 37 or 40 degrees C or from 20 degrees C to 33, 37, or 40 degrees C. Analysis of [35S]methionine-labeled Hsps by two-dimensional electrophoresis and autoradiography revealed 12 Hsps. Western immunoblot analysis with antisera to highly conserved Escherichia coli and Mycobacterium tuberculosis Hsps revealed a single 72-kilodalton (kDa) protein band that reacted with antibodies to E. coli DnaK and with antibodies to the M. tuberculosis 71-kDa Hsp homolog of E. coli DnaK. Two proteins with apparent molecular masses of 66 and 60 kDa reacted with antibodies against the M. tuberculosis 65-kDa Hsp homolog of E. coli GroEL. Human immune sera collected from patients with Lyme disease reacted with both the 66-kDa Hsp and the 60-kDa Hsp but failed to react with the 72-kDa Hsp. These data are discussed with regard to the possibility that host recognition of highly conserved epitopes of GroEL homologs of B. burgdorferi may result in autoimmune reactions causing arthritis and other pathologies.
针对伯氏疏螺旋体B31细胞产生的热休克蛋白（Hsps），对其热休克反应进行了表征。在用[35S]甲硫氨酸标记细胞后，经温度从33℃转变至37℃或40℃，或从20℃转变至33℃、37℃或40℃处理后，通过十二烷基硫酸钠 - 凝胶电泳和蛋白质荧光自显影检测到5至7种热休克蛋白。通过二维电泳和放射自显影分析[35S]甲硫氨酸标记的热休克蛋白，发现了12种热休克蛋白。使用针对高度保守的大肠杆菌和结核分枝杆菌热休克蛋白的抗血清进行的蛋白质免疫印迹分析显示，有一条单一的72千道尔顿（kDa）蛋白条带，它能与抗大肠杆菌DnaK的抗体以及抗结核分枝杆菌中大肠杆菌DnaK的71 kDa热休克蛋白同源物的抗体发生反应。两种表观分子量分别为66 kDa和60 kDa的蛋白质能与抗结核分枝杆菌中大肠杆菌GroEL的65 kDa热休克蛋白同源物的抗体发生反应。从莱姆病患者收集的人类免疫血清能与66 kDa热休克蛋白和60 kDa热休克蛋白发生反应，但不能与72 kDa热休克蛋白发生反应。针对伯氏疏螺旋体GroEL同源物高度保守表位的宿主识别可能导致自身免疫反应，进而引发关节炎和其他病理状况这一可能性，对这些数据进行了讨论。
