Marangoni Antonella, Aldini Rita, Sambri Vittorio, Giacani Lorenzo, Di Leo Korinne, Cevenini Roberto
Sezione di Microbiologia DMCSS, University of Bologna, Policlinico S Orsola, Via Massarenti 9, 40138 Bologna, Italy.
FEMS Immunol Med Microbiol. 2004 Apr 9;40(3):187-91. doi: 10.1016/S0928-8244(03)00345-6.
Stimulation of isolated rat Kupffer cells by viable Leptospira interrogans, Treponema pallidum and Borrelia garinii elicited cellular responses resulting in the release of different tumor necrosis factor alpha (TNF-alpha) levels, depending on the spirochetes. L. interrogans induced TNF-alpha levels higher than those achieved with B. garinii and T. pallidum (in this order), but lower than the levels achieved with lipopolysaccharide (LPS). In contrast to L. interrogans, pretreatment of borreliae and treponemes with polymyxin B did not substantially diminish the ability of B. garinii and T. pallidum to stimulate Kupffer cells. Purified T. pallidum lipoproteins TpN47, TmpA, TpN15-TpN17, and B. garinii OspA induced TNF-alpha responses comparable to that achieved by LPS. This response was almost insensitive to the action of polymyxin B.
活的问号钩端螺旋体、梅毒螺旋体和伽氏疏螺旋体对分离出的大鼠库普弗细胞的刺激引发了细胞反应，导致根据螺旋体的不同而释放出不同水平的肿瘤坏死因子α（TNF-α）。问号钩端螺旋体诱导的TNF-α水平高于伽氏疏螺旋体和梅毒螺旋体所达到的水平（按此顺序），但低于脂多糖（LPS）所达到的水平。与问号钩端螺旋体不同，用多粘菌素B对疏螺旋体和密螺旋体进行预处理并不会显著降低伽氏疏螺旋体和梅毒螺旋体刺激库普弗细胞的能力。纯化的梅毒螺旋体脂蛋白TpN47、TmpA、TpN15 - TpN17以及伽氏疏螺旋体OspA诱导的TNF-α反应与LPS所达到的反应相当。这种反应对多粘菌素B的作用几乎不敏感。
