Centre for Biomedical Sciences, Cardiff School of Health Sciences, University of Wales Institute Cardiff, Western Avenue, Cardiff CF5 2YB, UK.
Int Wound J. 2009 Dec;6(6):453-62. doi: 10.1111/j.1742-481X.2009.00640.x.
The complex interactions between patients and the microbial species that reside in their wounds are not yet fully characterised. Investigations to date have dealt with either those organisms that cause infections or those that establish long-term colonisations. The objective of this open, prospective pilot study was to quantitatively and qualitatively assess the microbiological status of 'locally infected' venous leg ulcers. Three different sampling techniques were used to recover organisms from 20 venous leg ulcers that had failed to progress towards healing and comparisons made to explore the distribution of colonised flora within the wound. In total, 116 isolates were recovered (mean of 5.8 per ulcer) with highest recovery rates seen with swabs. Greatest agreement of colonisation and counts was found between swabs and absorbent polyvinyl acetate (PVA) foam disc, and also between PVA discs and biopsies. Lowest agreement was between the distribution of anaerobes and coryneforms in swabs and biopsies, suggesting uneven vertical distribution within ulcers. No justification for using routine biopsies in locally infected venous leg ulcers was found.
患者与驻留在其伤口中的微生物之间的复杂相互作用尚未完全阐明。迄今为止的研究主要针对引起感染的生物体或那些建立长期定植的生物体。本开放性、前瞻性初步研究的目的是定量和定性评估“局部感染”静脉溃疡的微生物状况。使用三种不同的采样技术从 20 例未能愈合的静脉溃疡中回收微生物，并进行比较以探索定植菌群在伤口内的分布。总共回收了 116 株分离物（每个溃疡的平均值为 5.8 株），其中拭子的回收率最高。拭子和吸水性聚醋酸乙烯酯（PVA）泡沫盘之间以及 PVA 盘和活检之间的定植和计数一致性最大。在拭子和活检中，厌氧菌和棒状杆菌的分布之间的一致性最低，表明溃疡内存在不均匀的垂直分布。在局部感染的静脉溃疡中，没有理由使用常规活检。
