Beighton D, Decker J, Homer K A
Hunterian Dental Research Unit, London Hospital Medical College, Whitechapel, UK.
J Clin Periodontol. 1991 Feb;18(2):85-9. doi: 10.1111/j.1600-051x.1991.tb01693.x.
Chlorhexidine was tested for its ability to inhibit a wide range of glycosidic and proteolytic enzyme activities produced by Treponema denticola, Porphyromonas gingivalis, Bacteroides intermedius, Actinobacillus actinomycemcomitans, Capnocytophaga sputigena, Capnocytophaga gingivalis, Capnocytophaga orchracea, Capnocytophaga sp., Actinomyces viscosus, Streptococcus mitior, Streptococcus mutans, Streptococcus sobrinus, Streptococcus mitis, Streptococcus anginosus, Streptococcus oralis and Streptococcus sanguis. The enzymes produced by Capnocytophaga spp. were the most resistant to inhibition by chlorhexidine while the hydrolysis of proteolytic substrates by all the other species was markedly susceptible to inhibition with less than 0.125 mM chlorhexidine inhibiting enzyme activities by greater than or equal to 50%. Glycosidase activities, of all species, were generally more resistant to inhibition, especially neuraminidase activity. Chlorhexidine at less than 0.032 mM inhibited the degradation of bovine serum albumin by suspensions of dental plaque bacteria. These observations support an hypothesis that chlorhexidine exerts a bacteristatic effect in vivo, in part, by reducing the ability of dental plaque bacteria to degrade host-derived proteins and glycoproteins which normally provide essential nutrients for growth.
对洗必泰抑制多种糖苷酶和蛋白酶活性的能力进行了测试，这些酶由具核梭杆菌、牙龈卟啉单胞菌、中间普氏菌、伴放线放线杆菌、生痰二氧化碳嗜纤维菌、牙龈二氧化碳嗜纤维菌、黄褐二氧化碳嗜纤维菌、二氧化碳嗜纤维菌属、粘性放线菌、轻链球菌、变形链球菌、远缘链球菌、缓症链球菌、咽峡炎链球菌、口腔链球菌和血链球菌产生。二氧化碳嗜纤维菌属产生的酶对洗必泰的抑制作用最具抗性，而所有其他菌种对蛋白水解底物的水解作用则对抑制作用非常敏感，浓度低于0.125 mM的洗必泰就能使酶活性受到大于或等于50%的抑制。所有菌种的糖苷酶活性通常对抑制作用更具抗性，尤其是神经氨酸酶活性。浓度低于0.032 mM的洗必泰可抑制牙菌斑细菌悬液对牛血清白蛋白的降解。这些观察结果支持了这样一种假说，即洗必泰在体内发挥抑菌作用，部分原因是通过降低牙菌斑细菌降解宿主来源的蛋白质和糖蛋白的能力，而这些蛋白质和糖蛋白通常为细菌生长提供必需的营养物质。
