用于鉴定口腔源性拟杆菌属、嗜二氧化碳噬纤维菌属和螺旋体的API ZYM系统。
API ZYM system for identification of Bacteroides spp., Capnocytophaga spp., and spirochetes of oral origin.
作者信息
Laughon B E, Syed S A, Loesche W J
出版信息
J Clin Microbiol. 1982 Jan;15(1):97-102. doi: 10.1128/jcm.15.1.97-102.1982.
Abstract
A total of 80 oral strains of Bacteroides gingivalis, B. asaccharolyticus, B. melaninogenicus subsp. intermedius, B. melaninogenicus subsp. melaninogenicus, Capnocytophaga, Treponema denticola, and T. vincentii were characterized with the API ZYM system for 19 enzyme activities. Comparison of anaerobic and aerobic incubation with nine reference strains of these organisms showed no important differences. The key differential tests for black-pigmented Bacteroides strains and treponemes of oral origin were trypsin, alpha-glucosidase, and N-acetyl-beta-glucosaminidase. All Capnocytophaga strains produced distinctive aminopeptidase activities but varied in their glycosidic capabilities. The presence of a trypsin-like activity in B. gingivalis, T. denticola, and a group of Capnocytophaga strains may contribute to tissue destruction in periodontal disease.
摘要
总共80株牙龈拟杆菌、解糖拟杆菌、中间型产黑色素拟杆菌、产黑色素产黑色素拟杆菌、二氧化碳嗜纤维菌、具核梭杆菌和文森氏梭杆菌用API ZYM系统对19种酶活性进行了鉴定。这些微生物的9株参考菌株在厌氧和好氧培养条件下的比较显示没有重要差异。对口腔来源的产黑色素拟杆菌菌株和密螺旋体的关键鉴别试验是胰蛋白酶、α-葡萄糖苷酶和N-乙酰-β-葡萄糖胺酶。所有二氧化碳嗜纤维菌菌株都产生独特的氨肽酶活性，但糖苷酶能力各不相同。牙龈拟杆菌、具核梭杆菌和一组二氧化碳嗜纤维菌菌株中存在类胰蛋白酶活性可能导致牙周病中的组织破坏。
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J Clin Microbiol. 1982 Jan;15(1):97-102. doi: 10.1128/jcm.15.1.97-102.1982.
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本文引用的文献
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ELECTRON MICROSCOPIC OBSERVATIONS ON THE BACTERIAL FLORA OF ACUTE NECROTIZING ULCERATIVE GINGIVITIS.急性坏死性溃疡性龈炎细菌区系的电子显微镜观察
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Characteristics of Bacteroides asaccharolyticus from dental plaques of beagle dogs.来自比格犬牙菌斑的解糖拟杆菌的特性
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Detection of a fibroblast proliferation inhibitory factor from Capnocytophaga sputigena.从牙龈二氧化碳嗜纤维菌中检测成纤维细胞增殖抑制因子。
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J Clin Pathol. 1980 Jan;33(1):53-7. doi: 10.1136/jcp.33.1.53.
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Treatment of periodontal infections due to anaerobic bacteria with short-term treatment with metronidazole.使用甲硝唑短期治疗厌氧菌引起的牙周感染。
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