Suido H, Nakamura M, Mashimo P A, Zambon J J, Genco R J
Department of Oral Biology, School of Dental Medicine, University of New York at Buffalo 14214.
J Dent Res. 1986 Nov;65(11):1335-40. doi: 10.1177/00220345860650111101.
Protease and peptidase enzymes are thought to play a role in the virulence of many oral organisms, especially those associated with periodontal diseases. In order to evaluate the peptidases of periodontopathogens, we compared the arylaminopeptidase activities of Bacteroides gingivalis with those of other oral and non-oral bacteria. Sixty-three bacterial strains representing the prominent cultivable organisms in human periodontal pockets were tested, including representatives of the black-pigmented Bacteroides, Actinobacillus, Actinomyces, Campylobacter, Capnocytophaga, Eikenella, Fusobacterium, Haemophilus, Lactobacillus, Streptococcus, and Veillonella species. Each micro-organism was examined for its ability to hydrolyze 18 synthetic substrates of beta-naphthylamide derivatives of amino acids, dipeptides, and tripeptides. Quantitation of the enzyme activity was accomplished by colorimetric measurement of the amounts of released beta-naphthylamines. N-CBz-glycyl-glycyl-L-arginine-beta-naphthylamide was readily cleaved by B. gingivalis, but slightly or not at all by the other oral strains tested. L-arginine-beta-naphthylamide was cleaved by B. gingivalis, Capnocytophaga species, and Streptococcus species, but not readily by the other Bacteroides strains. Some dipeptide substrates tested, such as glycyl-L-arginine- and glycyl-L-proline-beta-naphthylamide, were strongly cleaved by B. gingivalis and weakly cleaved by other Bacteroides strains. Since high levels of N-CBz-glycyl-glycyl-L-arginyl-aminopeptidase activity are characteristic of B. gingivalis, its measurement may be valuable in the identification of this organism in clinical samples as an aid in diagnosis and monitoring of periodontal infections. Furthermore, this and other aminopeptidases produced by B. gingivalis and other oral organisms may play a role in the tissue destruction seen in periodontal disease.
蛋白酶和肽酶被认为在许多口腔微生物的毒力中起作用，尤其是那些与牙周疾病相关的微生物。为了评估牙周病原体的肽酶，我们比较了牙龈卟啉单胞菌与其他口腔和非口腔细菌的芳基氨基肽酶活性。测试了代表人类牙周袋中主要可培养微生物的63株细菌，包括黑色色素卟啉单胞菌、放线杆菌、放线菌、弯曲杆菌、二氧化碳嗜纤维菌、艾肯菌、梭杆菌、嗜血杆菌、乳酸杆菌、链球菌和韦荣球菌属的代表菌株。检测了每种微生物水解18种氨基酸、二肽和三肽的β-萘酰胺衍生物合成底物的能力。通过比色法测量释放的β-萘胺量来定量酶活性。N-CBz-甘氨酰-甘氨酰-L-精氨酸-β-萘酰胺很容易被牙龈卟啉单胞菌裂解，但被测试的其他口腔菌株轻微裂解或根本不裂解。L-精氨酸-β-萘酰胺被牙龈卟啉单胞菌、二氧化碳嗜纤维菌属和链球菌属裂解，但其他卟啉单胞菌菌株不容易裂解。一些测试的二肽底物，如甘氨酰-L-精氨酸-和甘氨酰-L-脯氨酸-β-萘酰胺，被牙龈卟啉单胞菌强烈裂解，而被其他卟啉单胞菌菌株弱裂解。由于高水平的N-CBz-甘氨酰-甘氨酰-L-精氨酰氨基肽酶活性是牙龈卟啉单胞菌的特征，其检测在临床样本中鉴定该微生物以辅助牙周感染的诊断和监测可能是有价值的。此外，牙龈卟啉单胞菌和其他口腔微生物产生的这种和其他氨基肽酶可能在牙周疾病中所见的组织破坏中起作用。
