Steacie Institute for Molecular Science, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario K1A0R6, Canada.
Biomaterials. 2010 Apr;31(10):2866-73. doi: 10.1016/j.biomaterials.2009.12.025. Epub 2010 Jan 6.
We have developed an ion-sensing nanoparticle that is comprised of a superparamagnetic iron oxide (SPIO) core encapsulated with a porous silica shell. The latter can be readily anchored with ligands capable of coordinating with positron-emitting metal. Evidently, this nanoparticle has a great potential for use in cell tracking with magnetic resonance (MR) imaging and positron emission tomography (PET). Herein we report the synthesis, surface functionalization and characterization of the magnetic nanoparticle-based probes and evaluate their cell-labeling efficacy, cytotoxicity and relaxivity in comparison to one of the most commonly utilized MRI contrast agents, Feridex.
我们开发了一种离子感应纳米颗粒，它由超顺磁性氧化铁 (SPIO) 核心和多孔硅壳组成。后者可以很容易地与能够与正电子发射金属配位的配体结合。显然，这种纳米颗粒在使用磁共振 (MR) 成像和正电子发射断层扫描 (PET) 进行细胞跟踪方面具有很大的潜力。在此，我们报告了基于磁性纳米颗粒的探针的合成、表面功能化和表征，并评估了它们的细胞标记效率、细胞毒性和弛豫率，与最常用的 MRI 造影剂之一 Feridex 进行了比较。
