Department of Neurosurgery, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
Contrast Media Mol Imaging. 2010 Jan-Feb;5(1):18-22. doi: 10.1002/cmmi.363.
In vivo molecular imaging is a rapidly growing research area both for basic and clinical science. Non-invasive imaging of in vivo conditions at the molecular level increases understanding of the biological characteristics of normal and diseased tissues without the need for invasive surgical procedures. Among the various imaging modalities, magnetic resonance imaging (MRI) has garnered interest as a molecular imaging modality due to its high spatial resolution. Here, we have demonstrated that the combined use of HER-2 targeting affibody, a small 7 kDa molecule that behaves similarly to antibodies, and superparamagnetic iron oxide (SPIO) can non-invasively image HER-2 expressing cells or tissues both in vitro and in vivo by MRI. This preliminary study demonstrates that affibody-SPIO is a feasible, target-specific contrast agent for in vivo MR molecular imaging.
体内分子成像在基础科学和临床医学领域都是一个快速发展的研究领域。在无需进行有创性手术的情况下，对分子水平的体内状态进行非侵入性成像，增加了对正常和病变组织的生物学特征的了解。在各种成像方式中，由于具有较高的空间分辨率，磁共振成像（MRI）已成为一种有吸引力的分子成像方式。在这里，我们已经证明，HER-2 靶向亲和体（一种类似抗体的小 7 kDa 分子）和超顺磁性氧化铁（SPIO）的联合使用可以通过 MRI 对体外和体内表达 HER-2 的细胞或组织进行非侵入性成像。这项初步研究表明，亲和体-SPIO 是一种可行的、针对特定靶点的体内 MR 分子成像对比剂。
